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WT1 antibody (AA 1-181)

WT1 Reactivity: Human IHC (f) Host: Mouse Monoclonal 6F-H2 unconjugated
Catalog No. ABIN6938782
  • Target See all WT1 Antibodies
    WT1 (Wilms Tumor 1 (WT1))
    Binding Specificity
    • 31
    • 16
    • 15
    • 6
    • 6
    • 6
    • 5
    • 5
    • 4
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 1-181
    Reactivity
    • 144
    • 67
    • 60
    • 17
    • 4
    • 4
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    Human
    Host
    • 82
    • 61
    • 1
    Mouse
    Clonality
    • 85
    • 59
    Monoclonal
    Conjugate
    • 70
    • 10
    • 5
    • 5
    • 5
    • 5
    • 5
    • 5
    • 5
    • 4
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This WT1 antibody is un-conjugated
    Application
    • 87
    • 81
    • 61
    • 56
    • 32
    • 21
    • 20
    • 14
    • 13
    • 5
    • 5
    • 4
    • 3
    • 2
    • 1
    • 1
    • 1
    Immunohistochemistry (Formalin-fixed Sections) (IHC (f))
    Specificity
    Recognizes a 47-55kDa-tumor suppressor protein, identified as Wilm's Tumor (WT1) protein. The antibody reacts with all isoforms of the full-length WT1 and also identifies WT1 lacking exon 2-encoded amino acids, frequently found in subsets of sporadic Wilm's tumors.WT1, a sporadic and familial pediatric kidney tumor, is genetically heterogeneous. Wilm's tumor is associated with mutations of WT1, a zinc-finger transcription factor that is essential for the development of the metanephric kidney and the urogenital system. The WT1 gene is normally expressed in fetal kidney and mesothelium, and its expression has been suggested as a marker for Wilm's tumor and mesothelioma. WT1 protein has been identified in proliferative mesothelial cells, malignant mesothelioma, ovarian carcinoma, gonadoblastoma, nephroblastoma, and desmoplastic small round cell tumor. Lung adenocarcinomas rarely stain positive with this antibody. WT1 protein expression in mesothelial cells has become a reliable marker for the diagnosis of mesotheliomas.
    Cross-Reactivity (Details)
    Human. Rat. Weakly reacts with Mouse.
    Purification
    1.0mg/ml of Ab purified from Bioreactor by Protein A/G.
    Immunogen
    Recombinant protein corresponding to residues 1-181 of human WT1.
    Clone
    6F-H2
    Isotype
    IgG1 kappa
    Top Product
    Discover our top product WT1 Primary Antibody
  • Application Notes

    Known_Application: Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes); Optimal dilution for a specific application should be determined.

    Positive_Control: K562 cells. Kidney, Testis, Wilm's Tumor or Mesothelioma.

    Restrictions
    For Research Use only
  • Concentration
    1.0 mg/mL
    Buffer
    Prepared in 10 mM PBS, WITHOUT BSA and Azide.
    Preservative
    Azide free
    Storage
    -20 °C,-80 °C
    Storage Comment
    Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.
    Expiry Date
    24 months
  • Target
    WT1 (Wilms Tumor 1 (WT1))
    Alternative Name
    WT1 (WT1 Products)
    Synonyms
    AWT1 antibody, EWS-WT1 antibody, GUD antibody, NPHS4 antibody, WAGR antibody, WIT-2 antibody, WT33 antibody, CWT1 antibody, WT1 antibody, XWT1a antibody, wt-1 antibody, wt1 antibody, xWT1 antibody, WT-1 antibody, gud antibody, wagr antibody, wt33 antibody, xwt1 antibody, wit-2 antibody, si:ch211-160j6.3 antibody, zgc:152697 antibody, D630046I19Rik antibody, Wt-1 antibody, XWT1b antibody, XeWT1 antibody, Wilms tumor 1 antibody, Wilms tumor 1 S homeolog antibody, wilms tumor 1b antibody, Wilms tumor protein antibody, Wilms tumor 1 homolog antibody, Wilms tumor 1 L homeolog antibody, WT1 antibody, wt1.S antibody, wt1 antibody, wt1b antibody, Wt1 antibody, wt1.L antibody
    Background
    WT1; AWT1; FWT1; GUD; NPHS4; WAGR; Wilms tumor 1,Wilm's Tumor 1 (WT1) (Wilm's Tumor & Mesothelial Marker)
    Cellular localisation: Nuclear and Cytoplasmic
    Molecular Weight
    47-55kDa
    Gene ID
    7490, 591980
    UniProt
    P19544
    Pathways
    Tube Formation
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