XRCC3 antibody

Catalog No. ABIN6938788

Product Details anti-XRCC3 Antibody

Target: XRCC3 (X-Ray Repair Complementing Defective Repair in Chinese Hamster Cells 3 (XRCC3))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This XRCC3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Specificity: The x-ray repair cross-complementing (XRCC) proteins are responsible for efficiently repairing and maintaining genetic stability following DNA base damage. These genes share sequence similarity with the yeast DNA repair protein Rad51. XRCC1 is a protein that facilitates the DNA base excision repair pathway by interacting with DNA ligase III and DNA polymerase to repair DNA single-strand breaks. XRCC2 and XRCC3 are both involved in maintaining chromosome stability during cell division. XRCC2 is required for efficient repair of DNA double-strand breaks by homologous recombination between sister chromatids, and XRCC3 interacts directly with Rad51 to cooperate with Rad51 during recombinational repair. XRCC4 is an accessory factor of DNA Ligase IV that preferentially binds DNA with nicks or broken ends. XRCC4 binds to DNA Ligase IV and enhances its joining activity, and it is also involved in V(D)J recombination. Any defect in one of the known components of the DNA repair/V(D)J recombination machinery (Ku-70, Ku-80, DNA-PKCS, XRCC4 and DNA Ligase IV) leads to abortion of the V(D)J rearrangement process and early block in both T and B cell maturation.

Cross-Reactivity (Details): Human.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Recombinant full-length human XRCC3 protein

Clone: 10F1-6

Isotype: IgG1 kappa

Application Details

Application Notes:

Known_Application: Western Blot (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 min at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.

Positive_Control: Human placenta. HeLa nuclear extract.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Expiry Date: 24 months

Target Details for XRCC3

Target: XRCC3 (X-Ray Repair Complementing Defective Repair in Chinese Hamster Cells 3 (XRCC3))

Alternative Name: XRCC3 (XRCC3 Products)

Background: CMM6, DNA repair protein XRCC3, RAD51 like, X ray repair complementing defective repair in Chinese hamster cells 3, X-ray repair cross-complementing protein 3, XRCC3,XRCC3
Cellular localisation: Nucleus and Cytoplasm.

Molecular Weight: 40kDa

Gene ID: 7517, 592325

UniProt: O43542

Pathways: DNA Damage Repair