YWHAE antibody

Catalog No. ABIN6938792

This Mouse Monoclonal antibody specifically detects YWHAE in WB and IHC. It exhibits reactivity toward Human, Mouse, Rat, Guinea Pig and Hamster.

Quick Overview for YWHAE antibody (ABIN6938792)

Target: YWHAE (14-3-3 epsilon (YWHAE))

Reactivity: Human, Mouse, Rat, Guinea Pig, Hamster

Host: Mouse

Clonality: Monoclonal

Conjugate: This YWHAE antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Clone: CPTC-YWHAE-1

Product Details anti-YWHAE Antibody

Purpose: 14-3-3E / Tryptophan 5-Monooxygenase Antibody

Specificity: Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathway. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. It interacts with CDC25 phosphatases, RAF1 and IRS1 proteins, suggesting its role in diverse biochemical activities related to signal transduction, such as cell division and regulation of insulin sensitivity. It has also been implicated in the pathogenesis of small cell lung cancer. The YWHAE gene which encodes 14-3-3E has also been identified as a possible susceptibility gene for schizophrenia.

Cross-Reactivity (Details): Human.

Purification: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Recombinant human full-length YWHAE protein

Isotype: IgG2a, kappa

Application Details

Application Notes:

Known_Application: Western Blot (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 min at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.

Positive_Control: HEK-293 or A-431 cell lines.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10  mM PBS

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for YWHAE

Target: YWHAE (14-3-3 epsilon (YWHAE))

Alternative Name: YWHAE

Background:

MDS, epididymis luminal protein 2, mitochondrial import stimulation factor (MSF) L subunit, Mitochondrial import stimulation factor L subunit, MSF L, protein kinase C inhibitor protein-1

HEK-293 or A-431 cell lines. Human Brain, Mouse Brain, Rat Brain, Hamster Brain, Guinea pig Brain, Human Lungs, Mouse Lungs, Rat Lungs, Hamster Lungs or Guinea pig Lungs

Molecular Weight: 29.4kDa

Gene ID: 7531, 513851

UniProt: P62258

Pathways: Neurotrophin Signaling Pathway, Myometrial Relaxation and Contraction, M Phase