ZFYVE28 antibody

Catalog No. ABIN6938800

The Mouse Monoclonal anti-ZFYVE28 antibody (Clone LST2-2426) (ABIN6938800) specifically detects ZFYVE28 in IHC (f). The antibody is reactive with Human samples.

Quick Overview for ZFYVE28 antibody (ABIN6938800)

Target: ZFYVE28 (Zinc Finger, FYVE Domain Containing 28 (ZFYVE28))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This ZFYVE28 antibody is un-conjugated

Application: Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Clone: LST2-2426

Product Details anti-ZFYVE28 Antibody

Specificity: ZFYVE28 belongs to the lst-2 family. It contains 1 FYVE-type zinc finger. The FYVE-type zinc finger mediates the interaction with phosphatidylinositol 3-phosphate (PI3P) and localization to early endosome membranes when not mono-ubiquitinated at Lys-87. Mono-ubiquitination at Lys-87 prevents binding to phosphatidylinositol 3-phosphate (PI3P) and localization to early endosome membranes. ZFYVE28 is a negative regulator of epidermal growth factor receptor (EGFR) signaling. It acts by promoting EGFR degradation in endosomes when not mono-ubiquitinated. The FYVE domain has been identified in a number of unrelated signaling molecules. This protein functions to recruit SMAD2 to the transforming growth factor-beta receptor. The FYVE domain is required to maintain the normal localization of this protein but is not involved in mediating interaction with SMADs.

Cross-Reactivity (Details): Human.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Recombinant full-length human ZFYVE28 protein

Isotype: IgG2a kappa

Application Details

Application Notes:

Known_Application: Immunohistochemistry (Formalin-fixed) (0.1-0.2 μg/mL for 30 min at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.

Positive_Control: PC3 cells. Testis, Brain, Colon, Kidney or Stomach.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Expiry Date: 24 months

Target Details for ZFYVE28

Target: ZFYVE28 (Zinc Finger, FYVE Domain Containing 28 (ZFYVE28))

Alternative Name: ZFYVE28

Background: Lateral signaling target protein 2 homolog, LST2, LYST2, Zinc finger FYVE domain-containing protein 28 (ZFYVE28),ZFYVE28 (Zinc Finger FYVE-type containing 28)
Cellular localisation: Cytoplasmic

Molecular Weight: 96kDa

Gene ID: 57732, 292056

Pathways: EGFR Signaling Pathway