ZFYVE28 antibody

Catalog No. ABIN6940433

The Mouse Monoclonal anti-ZFYVE28 antibody is suitable to detect ZFYVE28 in samples from Human. It has been validated for IHC.

Quick Overview for ZFYVE28 antibody (ABIN6940433)

Target: ZFYVE28 (Zinc Finger, FYVE Domain Containing 28 (ZFYVE28))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This ZFYVE28 antibody is un-conjugated

Application: Immunohistochemistry (IHC)

Clone: LST2-2426

Product Details anti-ZFYVE28 Antibody

Purpose: ZFYVE28 (Zinc Finger FYVE-type containing 28) Antibody

Specificity: ZFYVE28 belongs to the lst-2 family. It contains 1 FYVE-type zinc finger. The FYVE-type zinc finger mediates the interaction with phosphatidylinositol 3-phosphate (PI3P) and localization to early endosome membranes when not mono-ubiquitinated at Lys-87. Mono-ubiquitination at Lys-87 prevents binding to phosphatidylinositol 3-phosphate (PI3P) and localization to early endosome membranes. ZFYVE28 is a negative regulator of epidermal growth factor receptor (EGFR) signaling. It acts by promoting EGFR degradation in endosomes when not mono-ubiquitinated. The FYVE domain has been identified in a number of unrelated signaling molecules. This protein functions to recruit SMAD2 to the transforming growth factor-beta receptor. The FYVE domain is required to maintain the normal localization of this protein but is not involved in mediating interaction with SMADs.

Purification: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Recombinant full-length human LST2/ ZFYVE28 protein

Isotype: IgG2a, kappa

Application Details

Application Notes:

Positive Control: PC3 cells. Testis, Brain, Colon, Kidney or Stomach.

Known Application: Immunohistochemistry (Formalin-fixed) (0.1-0.2 μg/mL for 30 min at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for ZFYVE28

Target: ZFYVE28 (Zinc Finger, FYVE Domain Containing 28 (ZFYVE28))

Alternative Name: ZFYVE28

Background:

Lateral signaling target protein 2 homolog, LST2, LYST2, Zinc finger FYVE domain-containing protein 28 (ZFYVE28)

Brain, Colon, Kidney or Stomach., PC3 cells. Testis

Molecular Weight: 96kDa

Gene ID: 57732, 292056

UniProt: Q9HCC9

Pathways: EGFR Signaling Pathway