CLTA/CLTB antibody (N-Term)

Catalog No. ABIN6939151

The Mouse Monoclonal anti-CLTA/CLTB antibody is suitable to detect CLTA/CLTB in samples from Human. It has been validated for WB, IHC, FACS and IF.

Quick Overview for CLTA/CLTB antibody (N-Term) (ABIN6939151)

Target: CLTA/CLTB (Clathrin, Light Chain A/B (CLTA/CLTB))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CLTA/CLTB antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF)

Clone: SPM174

Product Details anti-CLTA/CLTB Antibody

Binding Specificity: N-Term

Purpose: Clathrin, Light Chain Antibody

Specificity: Recognizes proteins of 31-44 kDa, which are identified as Clathrin Light Chains (both A B). Clathrin is composed of three heavy chains and three light chains, which associate non-covalently to form a triskelion structure. Clathrin light chain regulates the self-assembly of triskelions onto intracellular membranes. Clathrin light chain subunits (LCA and LCB) contribute to regulation of coated vesicle formation to sort proteins during receptor-mediated endocytosis and organelle biogenesis. LCA and LCB are encoded by two discrete genes. They share only 60 % homology, and have certain features in common. Both LCA and LCB undergo alternative mRNA splicing, which results in the generation of tissue-specific isoforms.

Purification: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Purified recombinant N-terminal fragment of human Clathrin Light Chain

Isotype: IgG2b, kappa

Application Details

Application Notes:

Positive Control: HeLa cells. Placenta or Prostate Carcinoma.

Known Application: Flow Cytometry (0.5-1 μg/million cells), Immunofluorescence (1-2 μg/mL), Western Blot (0.5-1 μg/mL), Immunohistochemistry (Formalin-fixed) (0.5-1 μg/mL for 30 min at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for CLTA/CLTB

Target: CLTA/CLTB (Clathrin, Light Chain A/B (CLTA/CLTB))

Alternative Name: CLTA and CLTB

Background:

Clathrin light chain A, Clathrin light chain B, Clathrin light chain LCA, Clathrin light chain LCB, Clathrin light polypeptide A, Clathrin light polypeptide B, Clathrin, light polypeptide (Lca), Clathrin, light polypeptide (Lcb), CLTA, CLTB

HeLa cells. Placenta or Prostate Carcinoma.

Molecular Weight: 31-44kDa

Gene ID: 1211, 1212, 484241

UniProt: P09496, P09497