CTLA4 antibody

Catalog No. ABIN6939198

This anti-CTLA4 antibody is a Mouse Monoclonal antibody detecting CTLA4 in FACS, WB and IF. Suitable for Human, Mouse, Rat and Cow.

Quick Overview for CTLA4 antibody (ABIN6939198)

Target: CTLA4 (Cytotoxic T-Lymphocyte-Associated Protein 4 (CTLA4))

Reactivity: Human, Mouse, Rat, Cow

Host: Mouse

Clonality: Monoclonal

Conjugate: This CTLA4 antibody is un-conjugated

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunofluorescence (IF)

Clone: L4P2F5-F10

Product Details anti-CTLA4 Antibody

Purification: Purified by Protein A/G

Immunogen: A synthetic peptide from CTLA4 (exact sequence is proprietary)

Isotype: IgG1 kappa

Application Details

Application Notes:

Positive Control: Jurkat or Raji cells. Lymph Node or Tonsil.

Known Application: Flow Cytometry (0.5-1 μg/million cells), Immunofluorescence (1-2 μg/mL), Western Blot (0.5-1 μg/mL)Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for CTLA4

Target: CTLA4 (Cytotoxic T-Lymphocyte-Associated Protein 4 (CTLA4))

Alternative Name: CTLA4

Background: CTLA4 (CD152) is a cell surface receptor that behaves as a negative regulator of the proliferation and the effector function of T cells. It contains a V domain, a transmembrane domain, and a cytoplasmic tail. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. The membrane-bound isoform functions as a homodimer interconnected by a disulfide bond, while the soluble isoform functions as a monomer. Mutations in this gene have been associated with insulin-dependent diabetes mellitus, Graves disease, Hashimoto thyroiditis, celiac disease, systemic lupus erythematosus, thyroid-associated orbitopathy, and other autoimmune diseases.The novel finding that CTLA-4 Molecule is expressed and functional on human tumor cells opens up the possibility of antitumor therapeutic intervention based on targeting this molecule.

Molecular Weight: 30-43kDa

Gene ID: 1493

UniProt: P16410

Pathways: Cancer Immune Checkpoints