DBN1 antibody (AA 150-281)

Catalog No. ABIN6939227

The Mouse Monoclonal anti-DBN1 antibody (Clone DBN1-2879) (ABIN6939227) specifically detects DBN1 in WB and IHC. The antibody is reactive with Human samples.

Quick Overview for DBN1 antibody (AA 150-281) (ABIN6939227)

Target: DBN1 (Drebrin 1 (DBN1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This DBN1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Clone: DBN1-2879

Product Details anti-DBN1 Antibody

Binding Specificity: AA 150-281

Purpose: Drebrin 1 (DBN1) Antibody

Specificity: Drebrins (developmentally regulated brain proteins) are cytoplasmic proteins that bind F-actin in the brain and are involved in cell migration, extension of neuronal processes and plasticity of dendrites. There are three isoforms: two embryonic types (E1 and E2), and an adult type (A), generated by alternative RNA splicing from a single Drebrin gene. Drebrins are expressed mainly in brain neurons but are also found in skeletal muscle, heart, placenta, pancreas and kidney. Drebrin has been designated as a marker of the dendritic spine. Decreases in Drebrin levels in the brain have been been associated with Alzheimer s disease and Down syndrome.

Purification: 200ug/ml of Ab Purified from Bioreactor Concentrate.

Immunogen: Recombinant fragment (aa 150-281) of human DBN1 protein (exact sequence is proprietary)

Isotype: IgG1, kappa

Application Details

Application Notes:

Positive Control: Brain neurons. Also found in the heart, placenta, skeletal muscle, kidney and pancreas.

Known Application: Immunohistochemistry (Formalin-fixed) (0.5-1.0 μg/mL for 30 min at RT),(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for DBN1

Target: DBN1 (Drebrin 1 (DBN1))

Alternative Name: DBN1

Background:

Developmentally regulated brain protein, Drebrin 1, Drebrin, Drebrin E, Drebrin E2, Drebrin1, DrebrinE

kidney and pancreas., PC3 cells. Brain neurons. Also found in the heart, Placenta, Skeletal muscle

Molecular Weight: 120kDa

Gene ID: 1627, 130316

UniProt: Q16643

Pathways: Maintenance of Protein Location