Desmin antibody

Catalog No. ABIN6939233

The Mouse Monoclonal anti-Desmin antibody (Clone DES-1711) (ABIN6939233) specifically detects Desmin in WB, IHC, IF, FACS and StM. The antibody is reactive with Human, Rat, Mouse, Chicken and Hamster samples.

Quick Overview for Desmin antibody (ABIN6939233)

Target: Desmin (DES)

Reactivity: Human, Rat, Mouse, Chicken, Hamster

Host: Mouse

Clonality: Monoclonal

Conjugate: This Desmin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS), Staining Methods (StM)

Clone: DES-1711

Product Details anti-Desmin Antibody

Specificity: Cytoskeletal intermediate filaments (IFs) constitute a diverse group of proteins that are expressed in a highly tissue-specific manner. IFs are constructed from two-chain -helical coiled-coil molecules arranged on an imperfect helical lattice, and have been widely used as markers for distinguishing individual cell types within a tissue and identifying the origins of metastatic tumors. Vimentin is an IF general marker of cells originating in the mesenchyme. Vimentin and Desmin, a related class III IF, are both expressed during skeletal muscle development. Desmin, a 469 amino acid protein found near the Z line in sarcomeres, is expressed more frequently in adult differentiated state tissues. Anti-desmin detects cells of normal smooth, skeletal, and cardiac muscles.Antibody reacts with leiomyomas, leiomyosarcoma, rhabdomyomas, rhabdomyosarcoma, and perivascular cells of glomus tumors of the skin.

Purification: Purified by Protein A/G

Immunogen: Recombinant human desmin protein

Isotype: IgG1 kappa

Application Details

Application Notes:

Positive Control: SJRH30 cells. Uterus or Leiomyosarcoma.

Known Application: Immunofluorescence (1-2 μg/mL), Flow Cytometry (0.5-1 μg/million cells), Western Blot (0.5-1 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for Desmin

Target: Desmin (DES)

Alternative Name: DES

Molecular Weight: 52kDa

Gene ID: 1674

UniProt: P17661