Recombinant Desmin antibody

Catalog No. ABIN6939235

The Rabbit Monoclonal anti-Desmin antibody has been validated for IHC, StM and ISt. It is suitable to detect Desmin in samples from Human, Rat, Chicken and Hamster.

Quick Overview for Recombinant Desmin antibody (ABIN6939235)

Target: Desmin (DES)

Antibody Type: Recombinant Antibody

Reactivity: Human, Rat, Chicken, Hamster

Host: Rabbit

Clonality: Monoclonal

Conjugate: This Desmin antibody is un-conjugated

Application: Immunohistochemistry (IHC), Staining Methods (StM), Immunostaining (ISt)

Clone: DES-2960R

Product Details anti-Desmin Antibody

Specificity: Cytoskeletal intermediate filaments (IFs) constitute a diverse group of proteins that are expressed in a highly tissue-specific manner. IFs are constructed from two-chain -helical coiled-coil molecules arranged on an imperfect helical lattice, and have been widely used as markers for distinguishing individual cell types within a tissue and identifying the origins of metastatic tumors. Vimentin is an IF general marker of cells originating in the mesenchyme. Vimentin and Desmin, a related class III IF, are both expressed during skeletal muscle development. Desmin, a 469 amino acid protein found near the Z line in sarcomeres, is expressed more frequently in adult differentiated state tissues. Anti-desmin detects cells of normal smooth, skeletal, and cardiac muscles.Antibody reacts with leiomyomas, leiomyosarcoma, rhabdomyomas, rhabdomyosarcoma, and perivascular cells of glomus tumors of the skin.

Purification: Purified by Protein A/G

Immunogen: Recombinant human full-length DES protein

Isotype: IgG

Application Details

Application Notes:

Positive Control: SJRH30 cells. Uterus or Leiomyosarcoma.

Known Application: Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0 for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for Desmin

Target: Desmin (DES)

Alternative Name: DES

Molecular Weight: 52kDa

Gene ID: 1674

UniProt: P17661