ECM1 antibody

Catalog No. ABIN6939279

The Mouse Monoclonal anti-ECM1 antibody has been validated for IHC, IF, FACS and StM. It is suitable to detect ECM1 in samples from Human and Rat.

Quick Overview for ECM1 antibody (ABIN6939279)

Target: ECM1 (Extracellular Matrix Protein 1 (ECM1))

Reactivity: Human, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This ECM1 antibody is un-conjugated

Application: Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS), Staining Methods (StM)

Clone: SC05

Product Details anti-ECM1 Antibody

Specificity: This MAb reacts with a reduction-resistant epitope present in both free and SIgA bound Secretory Component. It does not react with the cell lines lacking secretory component. The antibody is useful for studying the distribution and level of both free and bound secretory component. Secretory component is differentially expressed in epithelium, and the antibody is a popular marker for identifying subpopulations of epithelial cells and epithelial differentiation. The Secretory component antibody is a useful research tool for studying mucosal immunity, inflammation, remodeling, differentiation and tumorigenesis, all processes associated with differential secretory component expression.

Purification: Purified by Protein A/G

Immunogen: Secretory Component protein isolated from human colostrum

Isotype: IgG1 kappa

Application Details

Application Notes:

Positive Control: Stomach, Lung or Breast tumor.

Known Application: Flow Cytometry (0.5-1 μg/million cells), Immunofluorescence (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (0.5-1 μg/mL for 30 minutes at RT) (Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for ECM1

Target: ECM1 (Extracellular Matrix Protein 1 (ECM1))

Alternative Name: ECM1

Molecular Weight: ~80kDa

Gene ID: 1893

UniProt: Q16610