TIGIT antibody (AA 22-141)

Catalog No. ABIN6939305

The Mouse Monoclonal anti-TIGIT antibody (Clone TIGIT-3017) (ABIN6939305) specifically detects TIGIT in IHC, FACS, ELISA, Coat and StM. The antibody is reactive with Human samples.

Quick Overview for TIGIT antibody (AA 22-141) (ABIN6939305)

Target: TIGIT (T Cell Immunoreceptor with Ig and ITIM Domains (TIGIT))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This TIGIT antibody is un-conjugated

Application: Immunohistochemistry (IHC), Flow Cytometry (FACS), ELISA, Coating (Coat), Staining Methods (StM)

Clone: TIGIT-3017

Product Details anti-TIGIT Antibody

Binding Specificity: AA 22-141

Purification: Purified by Protein A/G

Immunogen: Recombinant fragment (around aa 22-141) of human TIGIT protein (exact sequence is proprietary)

Isotype: IgG2c kappa

Application Details

Application Notes:

Positive Control: MOLT-4, Prostate or Colon Carcinoma.

Known Application: ELISA (For coating, order antibody without BSA), Flow Cytometry (1-2 μg/million cells),Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for TIGIT

Target: TIGIT (T Cell Immunoreceptor with Ig and ITIM Domains (TIGIT))

Alternative Name: TIGIT

Background: TIGIT is a checkpoint inhibitor which binds with high affinity to the poliovirus receptor (PVR), causing increased IL10 secretion, decreased IL12B secretion. TIGIT binding to PVR also causes the suppression of T cell activation by promoting the generation of mature immuno-regulatory dendritic cells. It is expressed at low levels on natural killer (NK) cells, as well as peripheral memory and regulatory CD4+ T cells. At the protein level, it is upregulated following the activation of these cells. Functionally, TIGIT is similar to CTLA4. The ligands for TIGIT include CD155 (signal abrogation) and CD226 (signal stimulation). It has been demonstrated to be upregulated on T cells in many cancers and is an immuno-oncology target for therapy.

Molecular Weight: 26kDa

Gene ID: 201633

Pathways: Cancer Immune Checkpoints