Recombinant EPO antibody (AA 28-162)

Catalog No. ABIN6939315

This Rabbit Monoclonal antibody specifically detects EPO in IHC, ELISA, Coat and StM. It exhibits reactivity toward Human.

Quick Overview for Recombinant EPO antibody (AA 28-162) (ABIN6939315)

Target: EPO (Erythropoietin (EPO))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This EPO antibody is un-conjugated

Application: Immunohistochemistry (IHC), ELISA, Coating (Coat), Staining Methods (StM)

Clone: EPO-3793R

Product Details anti-EPO Antibody

Binding Specificity: AA 28-162

Specificity: Recognizes a protein of about 37 kDa, which is identified as Erythropoietin (EPO). Erythropoietin is a secreted, glycosylated cytokine hormone composed of four alpha helical bundles. It is the primary factor responsible for regulating erythropoiesis during steady-state conditions and in response to blood loss and hemorrhage in the adult organism. Erythropoietin is synthesized by the kidney and stimulates the proliferation and maturation of bone marrow erythroid precursor cells. The protein is found in the plasma and regulates red cell production by promoting erythroid differentiation and initiating hemoglobin synthesis.

Purification: Purified by Protein A/G

Immunogen: Recombinant fragment (around aa 28-162) of human EPO protein (exact sequence is proprietary)

Isotype: IgG

Application Details

Application Notes:

Positive Control: HepG2 cells. Heart or Kidney.

Known Application: ELISA (Use Ab at 2-4 μg/mL for coating) (Order Ab without BSA), ,Immunohistochemistry (Formalin-fixed) (2-4 μg/mL for 30 min at RT),(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: 10mM PBS with 0.05% BSA & 0.05% azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for EPO

Target: EPO (Erythropoietin (EPO))

Alternative Name: EPO

Target Type: Hormone

Molecular Weight: 37kDa

Gene ID: 2056

UniProt: P01588

Pathways: JAK-STAT Signaling, Hormone Activity, Negative Regulation of intrinsic apoptotic Signaling, Negative Regulation of Transporter Activity