ErbB2/Her2 antibody (Extracellular Domain)

Catalog No. ABIN6939326

The Mouse Monoclonal anti-ErbB2/Her2 antibody has been validated for ELISA, IF, FACS and Coat. It is suitable to detect ErbB2/Her2 in samples from Human and Rat.

Quick Overview for ErbB2/Her2 antibody (Extracellular Domain) (ABIN6939326)

Target: ErbB2/Her2 (Receptor tyrosine-protein kinase erbB-2 (ErbB2/Her2))

Reactivity: Human, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This ErbB2/Her2 antibody is un-conjugated

Application: ELISA, Immunofluorescence (IF), Flow Cytometry (FACS), Coating (Coat)

Clone: HRB2-258

Product Details anti-ErbB2/Her2 Antibody

Binding Specificity: Extracellular Domain

Specificity: Recognizes a protein of 185 kDa, which is identified as c-erbB-2/HER-2/neu. Its epitope is localized in the extracellular domain. C-erbB-2/HER-2 is a member of the EGFR family. This MAb is specific and shows minimal cross-reaction with other members of the EGFR-family. Receptors of this family are located on the plasma membrane and consist of an extracellular ligand-binding domain that is connected to a large intracellular domain by a single transmembrane sequence. C-erbB-2/HER-2 protein is over-expressed in a variety of carcinomas especially those of breast and ovary.

Purification: Purified by Protein A/G

Immunogen: Recombinant extracellular domain of human HER-2 protein

Isotype: IgG2a kappa

Application Details

Application Notes:

Positive Control: SKBR-3 cells. Breast Cancers.

Known Application: ELISA (For coating, order antibody without BSA), ,Flow Cytometry (0.5-1 μg/million cells), ,Immunofluorescence (0.5-1 μg/mL), ,Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for ErbB2/Her2

Target: ErbB2/Her2 (Receptor tyrosine-protein kinase erbB-2 (ErbB2/Her2))

Alternative Name: ERBB2

Molecular Weight: 185kDa

Gene ID: 2064

Pathways: RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Skeletal Muscle Fiber Development