AKR1B1 antibody

Catalog No. ABIN6939403

The Mouse Monoclonal anti-AKR1B1 antibody (Clone CPTC-AKR1B1-3) (ABIN6939403) specifically detects AKR1B1 in WB, IHC and IF. The antibody is reactive with Human samples.

Quick Overview for AKR1B1 antibody (ABIN6939403)

Target: AKR1B1 (Aldo-Keto Reductase Family 1, Member B1 (Aldose Reductase) (AKR1B1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This AKR1B1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Clone: CPTC-AKR1B1-3

Product Details anti-AKR1B1 Antibody

Purpose: Aldo-keto Reductase Family 1 Member B1 (Adrenal Marker) Antibody

Specificity: AKR1B1, also designated as aldose reductase, is a member of the aldo/keto reductase superfamily, which consists of more than 40 known enzymes and proteins. This protein catalyzes the reduction of a number of aldehydes, including the aldehyde form of glucose, and is thereby implicated in the development of diabetic complications by catalyzing the reduction of glucose to sorbitol. It has also been shown to have decreased expression in adrenocortical cancer, and possibly play a role in adrenal tumorigenesis. It has been suggested that AKR1B1 could be investigated as a marker of malignancy for adrenal tumor diagnosis.

Purification: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Recombinant human full-length AKR1B1 protein

Isotype: IgG1, kappa

Application Details

Application Notes:

Positive Control: 293T, HEK293, A431, HeLa, HepG2, MOLT4, Jurkat and Raji whole cell lysates, Human Kidney, Human colon carcinoma tissue.

Known Application: Western Blot (1-2 μg/mL),Immunofluorescence (1-2 μg/mL),Immunohistochemistry (Formalin-fixed) (0.5-1 μg/mL for 30 min at RT),(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for AKR1B1

Target: AKR1B1 (Aldo-Keto Reductase Family 1, Member B1 (Aldose Reductase) (AKR1B1))

Alternative Name: AKR1B1

Background:

AKR1B1, Aldehyde reductase, aldehyde reductase 1, Aldo-keto reductase family 1 member B1, aldo-keto reductase family 1, member B1 (aldose reductase), Aldose reductase, AR, Lii5-2 CTCL tumor antigen, low Km aldose reductase

293T, HEK293, A431, HeLa, HePG2, Jurkat and Raji whole cell lysates, Human Kidney, Human colon carcinoma tissue., MOLT4

Molecular Weight: 37kDa

Gene ID: 231, 521212

UniProt: P15121

Pathways: Metabolism of Steroid Hormones and Vitamin D, C21-Steroid Hormone Metabolic Process, Monocarboxylic Acid Catabolic Process