Elastase 3B antibody (AA 82-238)

Catalog No. ABIN6939418

This Mouse Monoclonal antibody specifically detects Elastase 3B in WB, IHC, ELISA, Coat and StM. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for Elastase 3B antibody (AA 82-238) (ABIN6939418)

Target: Elastase 3B (ELA3B) (Elastase 3B, Pancreatic (ELA3B))

Reactivity: Human, Mouse, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This Elastase 3B antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Coating (Coat), Staining Methods (StM)

Clone: CELA3B-1257

Product Details anti-Elastase 3B Antibody

Binding Specificity: AA 82-238

Specificity: This MAb recognizes a protein of ~30 kDa, identified as CELA3B (Chymotrypsin like elastase family member 3B). Elastases form a subfamily of serine proteases that hydrolyze many proteins in addition to elastin. Humans have six elastase genes which encode the structurally similar proteins elastase 1, 2, 2A, 2B, 3A, and 3B. Unlike other elastases, elastase 3B has little elastolytic activity. Like most of the human elastases, elastase 3B is secreted from the pancreas as a zymogen and, like other serine proteases such as trypsin, chymotrypsin and kallikrein, it has a digestive function in the intestine. Elastase 3B preferentially cleaves proteins after alanine residues. Elastase 3B may also function in the intestinal transport and metabolism of cholesterol. Both elastase 3A and elastase 3B have been referred to as protease E and as elastase 1, and excretion of this protein in fecal material is frequently used as a measure of pancreatic function in clinical assays.

Purification: Purified by Protein A/G

Immunogen: Recombinant human CELA3B protein fragment (around aa 82-238) (exact sequence is proprietary)

Isotype: IgG1 kappa

Application Details

Application Notes:

Positive Control: Panc-28 or PANC1 cells. Pancreas.

Known Application: ELISA (Use Ab at 2-4 μg/mL for coating) (Order Ab without BSA)Western Blot (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes) Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for Elastase 3B

Target: Elastase 3B (ELA3B) (Elastase 3B, Pancreatic (ELA3B))

Alternative Name: CELA3B

Molecular Weight: ~17kDa

Gene ID: 23436

UniProt: P08861