GFAP antibody

Catalog No. ABIN6939502

This Mouse Monoclonal antibody specifically detects GFAP in WB, IHC, IF and FACS. It exhibits reactivity toward Human, Rat, Mouse, Cow, Pig, Rabbit, Chicken, Guinea Pig and Hamster.

Quick Overview for GFAP antibody (ABIN6939502)

Target: GFAP (Glial Fibrillary Acidic Protein (GFAP))

Reactivity: Human, Rat, Mouse, Cow, Pig, Rabbit, Chicken, Guinea Pig, Hamster

Host: Mouse

Clonality: Monoclonal

Conjugate: This GFAP antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)

Clone: GA-5

Product Details anti-GFAP Antibody

Purpose: GFAP (Astrocyte & Neural Stem Cell Marker) Antibody

Specificity: This MAb recognizes a protein of ~50 kDa which is identified as Glial Fibrillary Acidic Protein (GFAP). It shows no cross-reaction with other intermediate filament proteins. GFAP is specifically found in astroglia. GFAP is a very popular marker for localizing benign astrocyte and neoplastic cells of glial origin in the central nervous system. Antibody to GFAP is useful in differentiating primary gliomas from metastatic lesions in the brain and for documenting astrocytic differentiation in tumors outside the CNS.

Purification: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G.

Immunogen: GFAP isolated from pig spinal cord

Isotype: IgG1, kappa

Application Details

Application Notes:

Positive Control: T98G cells (FACS). Brain lysate (WB). Brain or Astrocytoma (IHC).

Known Application: Flow Cytometry (0.5-1 μg/million cells), Immunofluorescence (1-2 μg/mL), Western Blot (0.5-1 μg/mL), Immunohistochemistry (Formalin-fixed) (0.5-1.0 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for GFAP

Target: GFAP (Glial Fibrillary Acidic Protein (GFAP))

Alternative Name: GFAP

Background:

Astrocyte or Intermediate Filament Protein, Glial Fibrillary Acidic Protein (GFAP)

T98G cells (FACS). Brain or Astrocytoma (IHC). Human Brain, Mouse Brain, Rat Brain, Guinea Pig Brain or Hamster Brain tissue lysates (WB).

Molecular Weight: ~50kDa

Gene ID: 2670, 514227

UniProt: P14136