GPI antibody

Catalog No. ABIN6939548

This Mouse Monoclonal antibody specifically detects GPI in WB, IHC, IF and StM. It exhibits reactivity toward Human.

Quick Overview for GPI antibody (ABIN6939548)

Target: GPI (Glucose-6-Phosphate Isomerase (GPI))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This GPI antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Staining Methods (StM)

Clone: CPTC-GPI-1

Product Details anti-GPI Antibody

Purification: Purified by Protein A/G

Immunogen: Recombinant human full-length GPI protein

Isotype: IgG2a kappa

Application Details

Application Notes:

Positive Control: T47D, PC3, MCF-7, HepG2 and A549 cells. Brain, pancreas or thyroid carcinoma.

Known Application: Western Blot (1-2 μg/mL), Immunofluorescence (1-2 μg/mL),Immunohistochemistry (Formalin-fixed) (0.5-1 μg/mL for 30 min at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for GPI

Target: GPI (Glucose-6-Phosphate Isomerase (GPI))

Alternative Name: GPI

Background: Besides it's role as a glycolytic enzyme, mammalian GPI can function as a tumor-secreted cytokine and an angiogenic factor (AMF) that stimulates endothelial cell motility. GPI is also a neurotrophic factor (Neuroleukin) for spinal and sensory neurons. Defects in GPI are the cause of hemolytic anemia non-spherocytic due to glucose phosphate isomerase deficiency.

Molecular Weight: 55kDa

Gene ID: 2821

UniProt: P06744