TNC antibody

Catalog No. ABIN6939674

This Mouse Monoclonal antibody specifically detects TNC in IHC and StM. It exhibits reactivity toward Human.

Quick Overview for TNC antibody (ABIN6939674)

Target: TNC (Tenascin C (TNC))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This TNC antibody is un-conjugated

Application: Immunohistochemistry (IHC), Staining Methods (StM)

Clone: T2H5

Product Details anti-TNC Antibody

Specificity: In Western blotting, it reacts with two bands of ~MW of 210 kDa and 300 kDa, identified as two isoforms of Tenascin C. Specificity of this MAb is validated by sequential immunoprecipitation with a PAb against Tenascin C. Tenascin C is a multifunctional, disulfide-linkedhexameric extracellular matrix glycoprotein expressed in association with mesenchymal epithelial interactions during development and in the neo-vasculature and stroma of undifferentiated tumors. In adults, it is restricted to certain epithelial-stromal interfaces and increases markedly in hyper-proliferative diseases and in stroma of many neoplasms, including gliomas, breast, squamous and lung carcinomas.

No Cross-Reactivity: Rat (Rattus)

Purification: Purified by Protein A/G

Immunogen: Human breast carcinoma

Isotype: IgG1 kappa

Application Details

Application Notes:

Positive Control: Extracellular matrix in tonsil and blood vessels. Stroma of many tumors such as breast, squamous cell and lung carcinomas. Staining of normal fibroblasts serves as internal positive control.

Known Application: Immunohistochemistry (Formalin-fixed) (2-4 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes) ,Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for TNC

Target: TNC (Tenascin C (TNC))

Alternative Name: TNC

Molecular Weight: 210kDa and 300kDa

Gene ID: 3371

UniProt: P24821

Pathways: Regulation of Muscle Cell Differentiation, Regulation of Cell Size, Skeletal Muscle Fiber Development