ICAM-3/CD50 antibody

Catalog No. ABIN6939684

The Mouse Monoclonal anti-ICAM-3/CD50 antibody (Clone 101-1D2) (ABIN6939684) specifically detects ICAM-3/CD50 in FACS, IHC and IF. The antibody is reactive with Human samples.

Quick Overview for ICAM-3/CD50 antibody (ABIN6939684)

Target: ICAM-3/CD50 (ICAM3) (Intercellular Adhesion Molecule 3 (ICAM3))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This ICAM-3/CD50 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunofluorescence (IF)

Clone: 101-1D2

Product Details anti-ICAM-3/CD50 Antibody

Purpose: CD50 / ICAM3 Antibody

Specificity: Recognizes an N-glycosylated glycoprotein of 120 kDa with intra-chain disulfide bonds, identified as CD50 or ICAM-3 (WS: IV V). Its epitope localizes in the D2 extracellular domain and is resistant to neuraminidase and proteases. CD50 is the major ligand for LFA-1 (CD11a/CD18) and may have signalling role to increase adhesion. It is expressed on thymocytes and T lymphocytes and is resistant to treatment with phosphatidylinositol (PI) phospholipase C. This MAb inhibits primary mixed lymphocyte culture (MLC) but not secondary MLC, cytotoxicity or proliferation induced by mitogens. It blocks binding of NK1-L16 stimulated T cells to L cells expressing CD50. This MAb is excellent for staining of formalin/paraffin tissues.

Purification: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Stimulated human leukocytes

Isotype: IgG2a, kappa

Application Details

Application Notes:

Positive Control: HL-60 or THP-1 cells. Lymph node and tonsil.

Known Application: Flow Cytometry (0.5-1 μg/million cells), Immunofluorescence (0.5-1 μg/mL), Immunohistochemistry (Formalin-fixed) (0.5-1 μg/mL for 30 minutes at RT)(Staining of formalin/paraffin tissues Requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.)Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for ICAM-3/CD50

Target: ICAM-3/CD50 (ICAM3) (Intercellular Adhesion Molecule 3 (ICAM3))

Alternative Name: ICAM3

Background:

ICAMR, Intercellular adhesion molecule 3 (ICAM3)

HL-60 or THP-1 cells. Lymph node and tonsil.

Molecular Weight: 110-160kDa

Gene ID: 3385, 354563

UniProt: P32942