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ICAM-3/CD50 antibody

ICAM3 Reactivity: Human FACS, IF, IHC, IHC (p), StM Host: Mouse Monoclonal 101-1D2 unconjugated
Catalog No. ABIN6939684
  • Target See all ICAM-3/CD50 (ICAM3) Antibodies
    ICAM-3/CD50 (ICAM3) (Intercellular Adhesion Molecule 3 (ICAM3))
    Reactivity
    • 120
    • 3
    • 2
    • 2
    Human
    Host
    • 76
    • 43
    • 1
    Mouse
    Clonality
    • 82
    • 38
    Monoclonal
    Conjugate
    • 65
    • 9
    • 7
    • 5
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This ICAM-3/CD50 antibody is un-conjugated
    Application
    • 69
    • 48
    • 47
    • 29
    • 26
    • 26
    • 24
    • 15
    • 8
    • 7
    • 7
    • 6
    • 5
    • 4
    • 3
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Staining Methods (StM)
    Specificity
    Recognizes an N-glycosylated glycoprotein of 120 kDa with intra-chain disulfide bonds, identified as CD50 or ICAM-3 (WS: IV V). Its epitope localizes in the D2 extracellular domain and is resistant to neuraminidase and proteases. CD50 is the major ligand for LFA-1 (CD11a/CD18) and may have signalling role to increase adhesion. It is expressed on thymocytes and T lymphocytes and is resistant to treatment with phosphatidylinositol (PI) phospholipase C. This MAb inhibits primary mixed lymphocyte culture (MLC) but not secondary MLC, cytotoxicity or proliferation induced by mitogens. It blocks binding of NK1-L16 stimulated T cells to L cells expressing CD50. This MAb is excellent for staining of formalin/paraffin tissues.
    Purification
    Purified by Protein A/G
    Immunogen
    Stimulated human leukocytes
    Clone
    101-1D2
    Isotype
    IgG2a kappa
    Top Product
    Discover our top product ICAM3 Primary Antibody
  • Application Notes

    Positive Control: HL-60 or THP-1 cells. Lymph node and tonsil.

    Known Application: Flow Cytometry (0.5-1 μg/million cells), Immunofluorescence (0.5-1 μg/mL), Immunohistochemistry (Formalin-fixed) (0.5-1 μg/mL for 30 minutes at RT)(Staining of formalin/paraffin tissues Requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.)Optimal dilution for a specific application should be determined.

    Restrictions
    For Research Use only
  • Concentration
    200 μg/mL
    Buffer
    10 mM PBS with 0.05 % BSA & 0.05 % azide.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    4 °C,-80 °C
    Storage Comment
    Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
    Expiry Date
    24 months
  • Target
    ICAM-3/CD50 (ICAM3) (Intercellular Adhesion Molecule 3 (ICAM3))
    Alternative Name
    ICAM3 (ICAM3 Products)
    Molecular Weight
    110-160kDa
    Gene ID
    3385
    UniProt
    P32942
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