IGHG antibody

Catalog No. ABIN6939709

This Mouse Monoclonal antibody specifically detects IGHG in IHC, FACS, StM, IF and IHC (p). It exhibits reactivity toward Human.

Quick Overview for IGHG antibody (ABIN6939709)

Target: IGHG (Immunoglobulin Heavy Chain (Gamma Polypeptide) (IGHG))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This IGHG antibody is un-conjugated

Application: Immunohistochemistry (IHC), Flow Cytometry (FACS), Staining Methods (StM), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: IG217

Product Details anti-IGHG Antibody

Specificity: Recognizes a protein of 75 kDa, identified as gamma heavy chain of human immunoglobulins. It reacts with all sub-classes of gamma chain of human immunoglobulins. It does not cross-react with alpha (IgA), mu (IgM), epsilon (IgE), or delta (IgD), heavy chains, T-cells, monocytes, granulocytes, or erythrocytes. This MAb is useful in the identification of leukemias, plasmacytomas, and certain non-Hodgkin's lymphomas. The most common feature of these malignancies is the restricted expression of a single heavy chain class. Demonstration of clonality in lymphoid infiltrates indicates that the infiltrate is clonal and therefore malignant.

Purification: Purified by Protein A/G

Immunogen: Purified human Ig Gamma Chain

Isotype: IgG1 kappa

Application Details

Application Notes:

Positive Control: 293T, Raji or hPBL cells. Tonsil or Spleen.

Known Application: Flow Cytometry (0.5-1 μg/million cells), Immunofluorescence (0.5-1 μg/mL), Immunohistochemistry (Formalin/paraffin) (0.5-1 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for IGHG

Target: IGHG (Immunoglobulin Heavy Chain (Gamma Polypeptide) (IGHG))

Alternative Name: IGHG

Molecular Weight: 75kDa

Gene ID: 3500, 3501, 3502

UniProt: P01857, P01859, P01860, P01861