IGHM antibody

Catalog No. ABIN6939730

This Mouse Monoclonal antibody specifically detects IGHM in IHC, WB, StM, FACS and IF. It exhibits reactivity toward Human.

Quick Overview for IGHM antibody (ABIN6939730)

Target: IGHM (Immunoglobulin Heavy Constant mu (IGHM))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This IGHM antibody is un-conjugated

Application: Immunohistochemistry (IHC), Western Blotting (WB), Staining Methods (StM), Flow Cytometry (FACS), Immunofluorescence (IF)

Clone: IM373

Product Details anti-IGHM Antibody

Specificity: Recognizes a protein of 75 kDa, identified as mu heavy chain of human immunoglobulins. It does not cross-react with alpha (IgA), gamma (IgG), epsilon (IgE), or delta (IgD), heavy chains, T-cells, monocytes, granulocytes, or erythrocytes. This MAb is useful in the identification of leukemias, plasmacytomas, and certain non-Hodgkin's lymphomas. The most common feature of these malignancies is the restricted expression of a single heavy chain class. Demonstration of clonality in lymphoid infiltrates indicates that the infiltrate is clonal and therefore malignant.

Purification: Purified by Protein A/G

Immunogen: Recombinant full-length heavy chain of human IgM

Isotype: IgG2b kappa

Application Details

Application Notes:

Positive Control: 293T, Raji or hPBL cells. Tonsil or Spleen.

Known Application: Flow Cytometry (0.5-1 μg/million cells), Immunofluorescence (0.5-1 μg/mL), Western Blot (0.5-1 μg/mL), Immunohistochemistry (Formalin-fixed) (0.5-1 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for IGHM

Target: IGHM (Immunoglobulin Heavy Constant mu (IGHM))

Alternative Name: IGHM

Molecular Weight: 50-75kDa

Gene ID: 3507

UniProt: P01871, P20769