IgL antibody

Catalog No. ABIN6939758

This anti-IgL antibody is a Mouse Monoclonal antibody detecting IgL in IHC, FACS, IF, WB and StM. Suitable for Human.

Quick Overview for IgL antibody (ABIN6939758)

Target: IgL

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This IgL antibody is un-conjugated

Application: Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF), Western Blotting (WB), Staining Methods (StM)

Clone: HP6054

Product Details anti-IgL Antibody

Specificity: This MAb is specific to lambda light chain of immunoglobulin and shows no cross-reaction with lambda light chain or any of the five heavy chains. In mammals, the two light chains in an antibody are always identical, with only one type of light chain, kappa or lambda. The ratio of Kappa to Lambda is 70:30. However, with the occurrence of multiple myeloma or other B-cell malignancies this ratio is disturbed. Antibody to the lambda light chain is reportedly useful in the identification of leukemias, plasmacytomas, and certain non-Hodgkin's lymphomas. Demonstration of clonality in lymphoid infiltrates indicates that the infiltrate is malignant.

Purification: Purified by Protein A/G

Immunogen: Purified human IgG myeloma proteins coupled to polyaminostyren microbeads

Isotype: IgG2a kappa

Application Details

Application Notes:

Positive Control: 293T, Raji or hPBL cells. Tonsil or Spleen.

Known Application: Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Western Blot (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for IgL

Target: IgL

Molecular Weight: ~22.5kDa

Gene ID: 3535, 3546

UniProt: P01701, P01842