CD103 antibody (AA 775-855)

Catalog No. ABIN6939806

The Mouse Monoclonal anti-CD103 antibody is suitable to detect CD103 in samples from Human. It has been validated for IHC.

Quick Overview for CD103 antibody (AA 775-855) (ABIN6939806)

Target: CD103 (ITGAE) (Integrin, alpha E (Antigen CD103, Human Mucosal Lymphocyte Antigen 1, alpha Polypeptide) (ITGAE))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD103 antibody is un-conjugated

Application: Immunohistochemistry (IHC)

Clone: ITGAE-2063

Product Details anti-CD103 Antibody

Binding Specificity: AA 775-855

Purpose: CD103 / Integrin αEβ7 (T-Cell Lymphoma & Hairy Cell Leukemia Marker) Antibody

Specificity: This MAb recognizes a protein of 150 kDa, identified as CD103, which is the alpha-E integrin subunit of the heterodimeric alpha-E beta-7 (aEb7) integrin belonging to a small beta-7 integrin subfamily. CD103 is expressed on more than 95 % of intraepithelial CD8+ cells and on 40 % of mucosa-associated T cells, whereas less than 2 % of resting blood lymphocytes are CD103-positive. In several malignant conditions, such as T-cell lymphomas and hairy cell leukemia (HCL), the cells express CD103. Antibody to CD103 is an extremely useful addition to the IHC panel for the diagnosis of hairy cell leukemia (HCL).

Purification: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Recombinant human ITGAE/CD103 protein fragment (aa 775-855) (exact sequence is proprietary)

Isotype: IgG2c, kappa

Application Details

Application Notes:

Positive Control: Tonsil, Colon or Stomach.

Known Application: Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for CD103

Target: CD103 (ITGAE) (Integrin, alpha E (Antigen CD103, Human Mucosal Lymphocyte Antigen 1, alpha Polypeptide) (ITGAE))

Alternative Name: ITGAE

Background:

CD103, Human mucosal lymphocyte antigen 1 (HML1 antigen), Integrin alpha E heavy chain, Integrin alpha E light chain, Integrin alpha E, epithelial-associated, Integrin alpha E1, Integrin alpha-IEL (intestinal intraepithelial lymphocytes), Integrin alpha M290, Integrin alpha-E heavy chain, Integrin, alpha E (ITGAE)

Colon or Stomach, Tonsil

Molecular Weight: 150kDa

Gene ID: 3682, 513867

UniProt: P38570