Recombinant Integrin beta 3 antibody (AA 385-490)

Catalog No. ABIN6939828

This Mouse Monoclonal antibody specifically detects Integrin beta 3 in IHC and StM. It exhibits reactivity toward Human.

Quick Overview for Recombinant Integrin beta 3 antibody (AA 385-490) (ABIN6939828)

Target: Integrin beta 3 (ITGB3)

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Integrin beta 3 antibody is un-conjugated

Application: Immunohistochemistry (IHC), Staining Methods (StM)

Clone: RITGB3-2145

Product Details anti-Integrin beta 3 Antibody

Binding Specificity: AA 385-490

Specificity: Reacts with human integrin beta3 (GPIIIa, vitronectin receptor beta chain). It associates with the V-chain (CD51) to form vitronectin receptor, or with the IIb-chain (CD41) to form the GpIIb/GpIIIa complex (CD41/CD61).The CD41/CD61 complex appears early in megakaryocyte maturation. The activated CD41/CD61 complex is a receptor for von Willebrand factor, soluble fibrinogen, fibronectin, vitronectin and thrombospondin. It plays a central role in platelet activation and aggregation. The CD51/CD61 is implicated in tumor metastasis and adenoviral infection. The antibody detects platelets in smears of blood and bone marrow, as well as megakaryocytes in frozen sections and cell smears. The antibody is useful for classification of megakaryoblastic leukemia.

Purification: Purified by Protein A/G

Immunogen: Recombinant human ITGB3 protein fragment (aa 385-490) (exact sequence is proprietary)

Isotype: IgG1 kappa

Application Details

Application Notes:

Positive Control: Human spleen tissue (IHC).

Known Application: Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes) ,Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for Integrin beta 3

Target: Integrin beta 3 (ITGB3)

Alternative Name: ITGB3

Molecular Weight: 105kDa & 90kDa

Gene ID: 3690

UniProt: P05106

Pathways: Regulation of G-Protein Coupled Receptor Protein Signaling, Signaling Events mediated by VEGFR1 and VEGFR2, Smooth Muscle Cell Migration, Integrin Complex