Cytokeratin 1 antibody (C-Term)

Catalog No. ABIN6939860

This anti-Cytokeratin 1 antibody is a Mouse Monoclonal antibody detecting Cytokeratin 1 in IHC and StM. Suitable for Human and Rat.

Quick Overview for Cytokeratin 1 antibody (C-Term) (ABIN6939860)

Target: Cytokeratin 1 (KRT1) (Keratin 1 (KRT1))

Reactivity: Human, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This Cytokeratin 1 antibody is un-conjugated

Application: Immunohistochemistry (IHC), Staining Methods (StM)

Clone: LHK1

Product Details anti-Cytokeratin 1 Antibody

Binding Specificity: C-Term

Purification: Purified by Protein A/G

Immunogen: A 13-amino acid peptide (VRFVSTSYSGVTR) from the C-terminus of human KRT1 protein

Isotype: IgG2a kappa

Application Details

Application Notes:

Positive Control: Skin or Cervix.

Known Application: Immunohistochemistry (Formalin-fixed) (0.1-0.2 μg/mL for 30 min at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for Cytokeratin 1

Target: Cytokeratin 1 (KRT1) (Keratin 1 (KRT1))

Alternative Name: KRT1

Background: Keratins are a family of intermediate filament proteins that assemble into filaments through forming heterodimers of one type I keratin (keratins 9 to 23) and one type II keratin (keratins 1 to 8). Keratins demonstrate tissue and differentiation specific expression profiles. Cytokeratin 1 is a hetero-tetramer of two type I as well as two type II keratins, keratin-10 (KRT10). Keratin 1 is differentiation specific keratin that is one of the predominant keratins in suprabasal keratinocytes in stratified epithelia. Mutations in keratin 1 cause epidermolytic hyperkeratosis.

Molecular Weight: 67kDa

Gene ID: 38748

UniProt: P04264