Recombinant Cytokeratin 18 antibody

Catalog No. ABIN6939926

The Mouse Monoclonal anti-Cytokeratin 18 antibody has been validated for WB, IHC, IF, FACS and StM. It is suitable to detect Cytokeratin 18 in samples from Human, Mouse, Rat, Dog, Pig, Cat, Cow, Hamster, Sheep, Horse, Ferret, Goat and Marmoset.

Quick Overview for Recombinant Cytokeratin 18 antibody (ABIN6939926)

Target: Cytokeratin 18 (KRT18) (Keratin 18 (KRT18))

Antibody Type: Recombinant Antibody

Reactivity: Human, Mouse, Rat, Dog, Pig, Cat, Cow, Hamster, Sheep, Horse, Ferret, Goat, Marmoset

Host: Mouse

Clonality: Monoclonal

Conjugate: This Cytokeratin 18 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS), Staining Methods (StM)

Clone: RKRT18-1190

Product Details anti-Cytokeratin 18 Antibody

Specificity: This MAb reacts with a wide variety of simple epithelia. It does not react with stratified squamous epithelia. It reacts with epithelial tumors of the gastrointestinal tract, lung, breast, pancreas, ovary, and thyroid. Cytokeratin 18, which belongs to the type A (acidic) subfamily of low molecular weight keratins, exists in combination with cytokeratin 8. It was reported that tissues from gastrointestinal tract are positive for both cytokeratin 8 and 18 but do not contain cytokeratin 14. Tissues from gastrointestinal tract, respiratory tract and urogenital tract, as well as endocrine and exocrine tissues and mesothelial cells are positive for cytokeratin 18.

No Cross-Reactivity: Chicken, Xenopus laevis

Purification: Purified by Protein A/G

Immunogen: Cytoskeletal preparation extracted from the human vulvar squamous carcinoma cell line A431

Isotype: IgG1 kappa

Application Details

Application Notes:

Positive Control: MCF-7 or HeLa cells (FACS/IF). Human breast carcinoma tissue (IHC).

Known Application: Flow Cytometry (0.5-1 μg/million cells), Immunofluorescence (1-2 μg/mL), Western Blot (0.5-2 μg/mL), ,Immunohistochemistry (Formalin-fixed) (0.5-1 μg/mL for 30 min at RT),(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for Cytokeratin 18

Target: Cytokeratin 18 (KRT18) (Keratin 18 (KRT18))

Alternative Name: KRT18

Molecular Weight: 45kDa

Gene ID: 3875

UniProt: P05783

Pathways: Apoptosis, Caspase Cascade in Apoptosis