Recombinant LGALS1/Galectin 1 antibody (AA 12-108)

Catalog No. ABIN6939959

The Rabbit Monoclonal anti-LGALS1/Galectin 1 antibody has been validated for IHC, ELISA, Coat and StM. It is suitable to detect LGALS1/Galectin 1 in samples from Human.

Quick Overview for Recombinant LGALS1/Galectin 1 antibody (AA 12-108) (ABIN6939959)

Target: LGALS1/Galectin 1 (LGALS1) (Lectin, Galactoside-Binding, Soluble, 1 (LGALS1))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This LGALS1/Galectin 1 antibody is un-conjugated

Application: Immunohistochemistry (IHC), ELISA, Coating (Coat), Staining Methods (StM)

Clone: GAL1-2499R

Product Details anti-LGALS1/Galectin 1 Antibody

Binding Specificity: AA 12-108

Purification: Purified by Protein A/G

Immunogen: Recombinant fragment (around aa 12-108) of human Galectin-1 protein (exact sequence is proprietary)

Isotype: IgG

Application Details

Application Notes:

Positive Control: HeLa, K562 or 293 cells. Prostate, Kidney, Placenta, Stomach, Skin, Spleen, Brain or Heart.

Known Application: ELISA (Use Ab at 2-4 μg/mL for coating) (Order Ab without BSA), Immunohistochemistry (Formalin-fixed) (0.5-1 μg/mL for 30 min at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for LGALS1/Galectin 1

Target: LGALS1/Galectin 1 (LGALS1) (Lectin, Galactoside-Binding, Soluble, 1 (LGALS1))

Alternative Name: LGALS1

Background: Galectin-1 is a member of the beta-galactoside-binding family and is a dimeric protein of 14kD participating in a variety of normal and pathological processes, including cancer progression. Galectin-1 can affect the proliferation of normal and malignant cells. Inhibition of cell growth is observed in a lactose-dependent manner as lower concentrations of the lectin stimulate cell proliferation. Galectin-1 may also be implicated in the induction of apoptosis of activated T cells through the binding of exogenous galectin-1 to CD45 Molecules present on the surface of lymphocytes. Galectin-1, reported to be present either at the surface of cancer cells or accumulated around these cells could act as an immunological shield to protect against a T cell immune response and provide an advantage for survival.

Molecular Weight: 14kDa

Gene ID: 3956

UniProt: P09382

Pathways: Carbohydrate Homeostasis