CD99 antibody

Catalog No. ABIN6940046

This anti-CD99 antibody is a Rabbit Polyclonal antibody detecting CD99 in IHC and StM. Suitable for Human.

Quick Overview for CD99 antibody (ABIN6940046)

Target: CD99

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CD99 antibody is un-conjugated

Application: Immunohistochemistry (IHC), Staining Methods (StM)

Product Details anti-CD99 Antibody

Specificity: Recognizes a sialoglycoprotein of 27-32 kDa, identified as CD99, or MIC2 gene product, or E2 antigen. MIC2 gene is located in the pseudo-autosomal region of the human X and Y chromosome. MIC2 gene encodes two distinct proteins, which are produced by alternative splicing of the CD99 gene transcript and are identified as bands of 30 and 32 kDa (p30/32).Although its function is not fully understood, CD99 is implicated in various cellular processes including homotypic aggregation of T cells, upregulation of T cell receptor and MHS molecules, apoptosis of immature thymocytes and leukocyte diapedesis.CD99 is expressed on the cell membrane of some lymphocytes, cortical thymocytes, and granulosa cells of the ovary. Most pancreatic islet cells, Sertoli cells of the testis, and some endothelial cells express this antigen. Mature granulocytes express very little or no CD99. MIC2 is strongly expressed on Ewing's sarcoma cells and primitive peripheral neuroectodermal tumors.

Immunogen: Recombinant human MIC2 protein

Isotype: IgG

Application Details

Application Notes:

Positive Control: MOLT-4 cells. Pancreas or Ewing's sarcoma.

Known Application: Immunohistochemistry (Formalin-fixed) (5-10 μg/mL for 30 min at Room Temp)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for CD99

Target: CD99

Alternative Name: CD99

Molecular Weight: 27-32kDa

Gene ID: 4267

UniProt: P14209