Recombinant MITF antibody

Catalog No. ABIN6940051

The Rabbit Monoclonal anti-MITF antibody has been validated for IHC, IF, FACS and StM. It is suitable to detect MITF in samples from Human and Dog.

Quick Overview for Recombinant MITF antibody (ABIN6940051)

Target: MITF (Microphthalmia-Associated Transcription Factor (MITF))

Antibody Type: Recombinant Antibody

Reactivity: Human, Dog

Host: Rabbit

Clonality: Monoclonal

Conjugate: This MITF antibody is un-conjugated

Application: Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS), Staining Methods (StM)

Clone: MITF-2987R

Product Details anti-MITF Antibody

Specificity: MITF (microphthalmia transcription factor) is a basic helix-loop-helix-leucine-zipper (bHLH-Zip) transcription factor that regulates the development and survival of melanocytes and retinal pigment epithelium, and also is involved in transcription of pigmentation enzyme genes such as tyrosinase TRP1 and TRP2. MITF has been shown to be phosphorylated by MAP kinase in response to c-kit activation, resulting in upregulation of MITF transcriptional activity. Mutations of the MITF gene are associated with the autosomal dominant hereditary deafness and pigmentation condition, Waardenburg Syndrome type 2A. Multiple isoforms of MITF exist, including MITF-A, MITF-B, MITF-C, MITF-H, and MITF-M, which differ in the amino-terminal domain and in their expression patterns. The MITF-M isoform is restricted to the melanocyte cell lineage. This MAb recognizes a nuclear protein, which is expressed in the majority of primary and metastatic epithelioid malignant melanomas as well as in normal melanocytes, benign nevi and dysplastic nevi.

No Cross-Reactivity: Mouse (Murine), Rat (Rattus)

Purification: Purified by Protein A/G

Immunogen: Recombinant full-length human MiTF protein

Isotype: IgG

Application Details

Application Notes:

Positive Control: Jurkat, A-431, HeLa or 501 Mel human melanoma cells. Melanoma.

Known Application: Flow Cytometry (0.5-1 μg/million cells), Immunofluorescence (0.5-1 μg/mL), Immunohistochemistry (Formalin-fixed) (0.5-1.0 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for MITF

Target: MITF (Microphthalmia-Associated Transcription Factor (MITF))

Alternative Name: MITF

Molecular Weight: 52-56kDa (doublet)

Gene ID: 4286

UniProt: O75030

Pathways: Chromatin Binding