MTAP antibody (AA 97-196)

Catalog No. ABIN6940087

This Mouse Monoclonal antibody specifically detects MTAP in WB, IHC and ELISA. It exhibits reactivity toward Human.

Quick Overview for MTAP antibody (AA 97-196) (ABIN6940087)

Target: MTAP (Methylthioadenosine phosphorylase (MTAP))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This MTAP antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Clone: MTAP-1813

Product Details anti-MTAP Antibody

Binding Specificity: AA 97-196

Purpose: MTAP (Tumor Suppressor Marker) Antibody

Specificity: Recognizes a protein of 31 kDa, which is identified as MTAP (5'-deoxy-5'-methylthioadenosine phosphorylase). It catalyzes the reversible phosphorolysis of methylthioadenosine, which is important in polyamine metabolism and for the salvage of adenine and methionine. The gene encoding MTAP is linked to the tumor suppressor gene, p16INK4A. Deficient levels of MTAP can occur in cancers primarily through co-deletion of the MTAP gene and the p16INK4A gene. Cells expressing MTAP and possessing adenine salvage pathway activity may be less susceptible to malignancy due to growth-inhibitory actions of agents (e.g. antifolates), whose mechanism of action, in part, involves this de novo purine pathway.

Purification: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Recombinant human MTAP protein fragment (aa97-196) (exact sequence is proprietary)

Isotype: IgG2b, kappa

Application Details

Application Notes:

Positive Control: HepG2, A431, HeLa, HAP1 or MCF-7 cells. Kidney.

Known Application: ELISA (Use Ab at 2-4 μg/mL for coating) (Order Ab without BSA), Western Blot (1-2 μg/mL),Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for MTAP

Target: MTAP (Methylthioadenosine phosphorylase (MTAP))

Alternative Name: MTAP

Background:

BDMF, DMSFH, DMSMFH, Epididymis luminal protein 249, HEL249, LGMBF, MeSAdo phosphorylase, Methylthioadenosine phosphorylase, MSAP, MTA phosphorylase, MTAP, MTAPase, S-methyl-5''-thioadenosine phosphorylase

A431, HAP1 or MCF-7 cells. Kidney., HeLa, HePG2

Molecular Weight: 31kDa

Gene ID: 4507, 193268

UniProt: Q13126

Pathways: Ribonucleoside Biosynthetic Process, Methionine Biosynthetic Process