Recombinant Nkx2-2 antibody

Catalog No. ABIN6940203

This Mouse Monoclonal antibody specifically detects Nkx2-2 in IHC and StM. It exhibits reactivity toward Human, Mouse, Rat and Chicken.

Quick Overview for Recombinant Nkx2-2 antibody (ABIN6940203)

Target: Nkx2-2 (NK2 Homeobox 2 (Nkx2-2))

Antibody Type: Recombinant Antibody

Reactivity: Human, Mouse, Rat, Chicken

Host: Mouse

Clonality: Monoclonal

Conjugate: This Nkx2-2 antibody is un-conjugated

Application: Immunohistochemistry (IHC), Staining Methods (StM)

Clone: RNX2-294

Product Details anti-Nkx2-2 Antibody

Specificity: Expression of NKX2.2 has been found in neuroendocrine tumors of the gut, making it a potential marker for the study of gastrointestinal neuroendocrine tumors. More recently, NKX2.2 protein was identified as a target of EWS-FLI-1, the fusion protein specific to Ewing sarcoma, and was shown to be differentially upregulated in Ewing sarcoma on the basis of array-based gene expression analysis. It acts as a valuable marker for Ewing sarcoma, with a sensitivity of 93 % and a specificity of 89 %, and aids in the differential diagnosis of small round cell tumors.

Purification: Purified by Protein A/G

Immunogen: Recombinant full-length human NKX2.2 protein

Isotype: IgG1 kappa

Application Details

Application Notes:

Positive Control: Pancreas or Ewing's sarcoma.

Known Application: Immunohistochemistry (Formalin-fixed) (0.5-1 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for Nkx2-2

Target: Nkx2-2 (NK2 Homeobox 2 (Nkx2-2))

Alternative Name: NKX2-2

Molecular Weight: 40-50kDa

Gene ID: 4821

UniProt: O95096

Pathways: Dopaminergic Neurogenesis