Prohibitin antibody (AA 167-261)

Catalog No. ABIN6940301

The Mouse Monoclonal anti-Prohibitin antibody has been validated for WB, IHC, IF and StM. It is suitable to detect Prohibitin in samples from Human.

Quick Overview for Prohibitin antibody (AA 167-261) (ABIN6940301)

Target: Prohibitin (PHB)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Prohibitin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Staining Methods (StM)

Clone: PHB-3231

Product Details anti-Prohibitin Antibody

Binding Specificity: AA 167-261

Specificity: Recognizes a protein of 30 kDa which is identified as Prohibitin, an evolutionarily conserved protein with homologues found in yeast to man. It is located in the inner membrane of mitochondria. Although prohibitin mRNA and protein expression occurs throughout the cell cycle, maximum levels are detected during the G1/S phase transition and minimum levels are seen in S phase and the G2/mitosis boundary. Prohibitin is located exclusively in the mitochondria with the highest concentration on the inner membrane. Prohibitin is an ideal mitochondrial marker. It shows antiproliferative activity and has been proposed to play a role in normal cell cycle regulation, replicative senescence, cellular immortalization, and tumor suppression.

Purification: Purified by Protein A/G

Immunogen: Recombinant human PHB protein fragment (aa167-261) (exact sequence is proprietary)

Isotype: IgG1 kappa

Application Details

Application Notes:

Positive Control: Ramos, Jurkat, HEK293, K562, HepG2, A431 or MCF-7 cells (IF). Liver, Kidney, Heart or Endometrium (IHC).

Known Application: Western Blot (1-2 μg/mL), Immunofluorescence (1-2 μg), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for Prohibitin

Target: Prohibitin (PHB)

Alternative Name: PHB

Molecular Weight: 30kDa

Gene ID: 5245

UniProt: P35232

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling