UACA antibody

Catalog No. ABIN6940368

This Mouse Monoclonal antibody specifically detects UACA in WB, IF and FACS. It exhibits reactivity toward Human and Mouse.

Quick Overview for UACA antibody (ABIN6940368)

Target: UACA (Uveal Autoantigen With Coiled Coil Domains And Ankyrin Repeats (UACA))

Reactivity: Human, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This UACA antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS)

Clone: AE-5

Product Details anti-UACA Antibody

Purpose: UACA / Nucling (Nuclear Membrane Marker) Antibody

Specificity: UACA (Uveal Autoantigen with Coiled-coil domains and Ankyrin repeats) is a 1,416 amino acid nuclear membrane protein. It was originally identified as an autoantigen in patients with panuveitis, a characteristic of Vogt-Koyanagi-Harada disease, and in patients with Graves' disease. UACA was also later identified as Nucling, an mRNA differentially expressed in F9 embryonal carcinoma cells during cardiac muscle differentiation. UACA appears to function as a pro-apoptotic protein that recruits the apaf-1-pro-caspase-9 complex for the induction of apoptosis to mediate the cell-death pathway.

Purification: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Nuclei of myeloid leukemia biopsy cells

Isotype: IgG1, kappa

Application Details

Application Notes:

Positive Control: HeLa or 293T cells. Highly expressed in skeletal muscle, heart, kidney and pancreas. Also expressed in epidermal melanocytes, eye muscles and thyroid follicular cells.

Known Application: Flow Cytometry (0.5-1 μg/million cells), Immunofluorescence (0.5-1.0 μg/mL), Western Blot (0.5-1 μg/mL),Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for UACA

Target: UACA (Uveal Autoantigen With Coiled Coil Domains And Ankyrin Repeats (UACA))

Alternative Name: UACA

Background:

Nuclear membrane binding protein, Uveal autoantigen with coiled-coil domains and ankyrin repeats (UACA), NUCLING

Highly expressed in skeletal muscle, kidney and pancreas. Also expressed in epidermal melanocytes, eye muscles and thyroid follicular cells. HeLa or 293T cells.

Molecular Weight: 160kDa

Gene ID: 55075, 108049

UniProt: Q9BZF9

Pathways: Positive Regulation of Endopeptidase Activity