RAD51 antibody (AA 1-134)

Catalog No. ABIN6940476

This Mouse Monoclonal antibody specifically detects RAD51 in IHC, FACS, ISt and StM. It exhibits reactivity toward Human.

Quick Overview for RAD51 antibody (AA 1-134) (ABIN6940476)

Target: RAD51 (DNA Repair Protein Homolog 1 (RAD51))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This RAD51 antibody is un-conjugated

Application: Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunostaining (ISt), Staining Methods (StM)

Clone: RAD51-2702

Product Details anti-RAD51 Antibody

Binding Specificity: AA 1-134

Purification: Purified by Protein A/G

Immunogen: Recombinant fragment of human RAD51 protein (around aa 1-134) (exact sequence is proprietary)

Isotype: IgG1 kappa

Application Details

Application Notes:

Positive Control: K562, HeLa, MCF-7 cells. Colon or breast Carcinoma.

Known Application: Flow Cytometry (1-2 μg/mL),Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for RAD51

Target: RAD51 (DNA Repair Protein Homolog 1 (RAD51))

Alternative Name: RAD51

Background: RAD51 is one of the key factors of DNA repair by homologous recombination and has been shown to have anti-apoptotic activity in tumor cells. RAD51 protein interacts with a variety of tumor suppressor proteins including p53, BRCA1 and BRCA2. Elevated expression of RAD51 enhances radio-resistance of human tumor cells. Overexpression of RAD51 protein in tumor cells renders them resistant against cytotoxic drugs like Cisplatin. RAD51 interacts with BRCA1 and BRCA2 to influence subcellular localization and cellular response to DNA damage. BRCA2 inactivation may be a key event leading to genomic instability and tumorigenesis from deregulation of RAD51. High-level expression of RAD51 has been observed in a variety of human malignancies.RAD51 overexpression correlates with histological grading of the tumor in invasive ductal mammary carcinoma.

Molecular Weight: 37kDa

Gene ID: 5888

UniProt: Q06609

Pathways: DNA Damage Repair