SERPINB3 antibody

Catalog No. ABIN6940565

The Mouse Monoclonal anti-SERPINB3 antibody (Clone CPTC-SERPINB3-2) (ABIN6940565) specifically detects SERPINB3 in WB. The antibody is reactive with Human samples.

Quick Overview for SERPINB3 antibody (ABIN6940565)

Target: SERPINB3 (serpin Peptidase Inhibitor, Clade B (Ovalbumin), Member 3 (SERPINB3))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This SERPINB3 antibody is un-conjugated

Application: Western Blotting (WB)

Clone: CPTC-SERPINB3-2

Product Details anti-SERPINB3 Antibody

Purpose: Squamous Cell Carcinoma Antigen 1 Antibody

Specificity: Metastasis of a primary tumor to a distant site is determined through signaling cascades that break down interactions between the cell and extracellular matrix proteins. Among the proteins mediating metastasis are serine proteases, such as neutrophil elastase. Serpins are family of serine protease inhibitors, contain a stretch of peptide that mimics a true substrate for a corresponding serine protease. Serine proteases bind to this substrate mimic in a 1:1 stoichiometric fashion and become catalytically inactive. Aberrant expression of serpin family members can contribute to a number of conditions, including emphysema (-1 antitrypsin deficiency), fatal bleeding (elastase to Thrombin specificity) and thrombosis (antithrombin deficiency), and are indicators of cancer stage phenotypes (circulating levels of squamous cell carcinoma antigen, known as SCCA1, increase in advancing stages of some cervical, lung, esophageal and head and neck cancers). SCCA1 expression has been demonstrated to promote oncogenic transformation and epithelial-mesenchymal transition (EMT) in mammary epithelial cells, and its silencing in breast cancer cells has been shown to halt their proliferation.

Purification: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Recombinant human full-length SERPINB3 protein

Isotype: IgG1, kappa

Application Details

Application Notes:

Positive Control: U-2197, HaCaT, Squamous cells. Expressed in some hepatocellular carcinoma.

Known Application: Western Blot (0.5-1.0 μg/mL), Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for SERPINB3

Target: SERPINB3 (serpin Peptidase Inhibitor, Clade B (Ovalbumin), Member 3 (SERPINB3))

Alternative Name: SERPINB3

Background:

HsT1196, Protein T4 A, Protein T4-A, SCC, SCCA-PD, SCCA1, SCCAPD, Serine (or cysteine) proteinase inhibitor clade B (ovalbumin) member 3, Serpin B3, SERPINB3, Squamous cell carcinoma antigen 1, T4 A, T4-A

A-431, HaCaT, Squamous cells. Expressed in some hepatocellular carcinoma., U-2197

Molecular Weight: 39kDa

Gene ID: 6317, 227948

UniProt: P29508