BMI1 antibody (AA 142-326)

Catalog No. ABIN6940580

The Mouse Monoclonal anti-BMI1 antibody has been validated for WB, IHC and StM. It is suitable to detect BMI1 in samples from Human.

Quick Overview for BMI1 antibody (AA 142-326) (ABIN6940580)

Target: BMI1 (BMI1 Polycomb Ring Finger Oncogene (BMI1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This BMI1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Staining Methods (StM)

Clone: BMI1-2689

Product Details anti-BMI1 Antibody

Binding Specificity: AA 142-326

Purification: Purified by Protein A/G

Immunogen: Recombinant fragment of human BMI1 protein (around aa 142-326) (exact sequence is proprietary)

Isotype: IgG1 kappa

Application Details

Application Notes:

Positive Control: 293T, NIH3T3, HeLa or Jurkat cells. Colon Carcinoma.

Known Application: Western Blot (1-2 μg/mL),Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for BMI1

Target: BMI1 (BMI1 Polycomb Ring Finger Oncogene (BMI1))

Alternative Name: BMI1

Background: The B cell-specific moloney murine leukemia virus integration site 1 (Bmi-1) is a transcriptional receptor of the polycomb gene family involved in several cellular processes including cell-cycle regulation, apoptosis, and maintenance of adult and neoplastic stem cells by providing self-renewal capacity. Further, Bmi-1 expression has been associated with malignant transformation, tumor progression, metastatic disease, and poor prognosis in human malignancies.

Molecular Weight: 41kDa

Gene ID: 648

UniProt: P35226

Pathways: Cell Division Cycle, Autophagy