Melanoma gp100 antibody
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- Target See all Melanoma gp100 (PMEL) Antibodies
- Melanoma gp100 (PMEL) (Premelanosome Protein (PMEL))
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Reactivity
- Human, Horse
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This Melanoma gp100 antibody is un-conjugated
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Application
- Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (IHC), Staining Methods (StM)
- Specificity
- By immunohistochemistry, it specifically recognizes a protein in melanocytes and melanomas. This MAb reacts with junctional and blue nevus cells and variably with fetal and neonatal melanocytes. Intradermal nevi, normal adult melanocytes, and non-melanocytic cells are negative. It does not stain tumor cells of epithelial, lymphoid, glial, or mesenchymal origin.This Mab labels formalin-fixed, paraffin-embedded melanomas and other tumors showing melanocytic differentiation.
- No Cross-Reactivity
- Rat (Rattus)
- Purification
- Purified by Protein A/G
- Immunogen
- Membranes from a human melanoma metastasis
- Clone
- SPM286
- Isotype
- IgG2b kappa
- Top Product
- Discover our top product PMEL Primary Antibody
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anti-Premelanosome Protein (PMEL) antibody
PMEL Reactivity: Human, Rat, Dog, Monkey IF, IHC, WB, IHC (p) Host: Mouse Monoclonal 10E5 unconjugated
anti-Premelanosome Protein (PMEL) (AA 376-502) antibodyPMEL Reactivity: Human IHC, ELISA, StM, Coat Host: Mouse Monoclonal PMEL-2037 unconjugated
anti-Premelanosome Protein (PMEL) (C-Term) antibodyVerified PMEL Reactivity: Human, Mouse IF, WB, ELISA Host: Goat Polyclonal unconjugated
anti-Premelanosome Protein (PMEL) antibodyPMEL Reactivity: Human IF, FACS, IHC, StM Host: Mouse Monoclonal HMB45 unconjugated
anti-Premelanosome Protein (PMEL) (N-Term) antibodyPMEL Reactivity: Human, Horse, Rat, Mouse, Dog, Cow, Goat, Guinea Pig, Rabbit IHC, WB Host: Rabbit Polyclonal unconjugated
anti-Premelanosome Protein (PMEL) (AA 25-467) antibodyPMEL Reactivity: Human IHC, WB, ELISA Host: Rabbit Polyclonal unconjugated
anti-Premelanosome Protein (PMEL) (C-Term) antibodyPMEL Reactivity: Human, Mouse IF, IHC, WB, ELISA, ICC Host: Rabbit Polyclonal unconjugated
anti-Premelanosome Protein (PMEL) antibodyPMEL Reactivity: Human IHC, StM Host: Rabbit Monoclonal PMEL-1825R unconjugated Recombinant Antibody
anti-Premelanosome Protein (PMEL) antibodyPMEL Reactivity: Human, Rat, Mouse IHC, WB Host: Rabbit Polyclonal unconjugated
anti-Premelanosome Protein (PMEL) antibodyPMEL Reactivity: Human IHC Host: Rabbit Monoclonal MSVA-617R unconjugated Recombinant Antibody
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- Application Notes
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Positive Control: SK-MEL-28 cells. Melanoma.
Known Application: Flow Cytometry (0.5-1 μg/million cells), Immunofluorescence (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.
- Restrictions
- For Research Use only
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- Concentration
- 200 μg/mL
- Buffer
- 10 mM PBS with 0.05 % BSA & 0.05 % azide.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C,-80 °C
- Storage Comment
- Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
- Expiry Date
- 24 months
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- Target
- Melanoma gp100 (PMEL) (Premelanosome Protein (PMEL))
- Alternative Name
- SILV (PMEL Products)
- Molecular Weight
- 90-100kDa
- Gene ID
- 6490
- UniProt
- P40967
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