SOX4 antibody

Catalog No. ABIN6940617

The Mouse Monoclonal anti-SOX4 antibody has been validated for IHC and StM. It is suitable to detect SOX4 in samples from Human.

Quick Overview for SOX4 antibody (ABIN6940617)

Target: SOX4 (SRY (Sex Determining Region Y)-Box 4 (SOX4))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This SOX4 antibody is un-conjugated

Application: Immunohistochemistry (IHC), Staining Methods (StM)

Clone: SOX4-2540

Product Details anti-SOX4 Antibody

Purification: Purified by Protein A/G

Immunogen: Recombinant full-length human SOX4 protein

Isotype: IgG2b kappa

Application Details

Application Notes:

Positive Control: Breast or Colon Carcinoma.

Known Application: Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for SOX4

Target: SOX4 (SRY (Sex Determining Region Y)-Box 4 (SOX4))

Alternative Name: SOX4

Background: SOX4 is a member of the SOX (SRY-related HMG-box) family of transcription factors with a critical role in embryonic development and in cell-fate determination during organogenesis of the heart, pancreas, brain, and in B and T lymphocyte differentiation. SOX4 gene expression is upregulated in many cancer types, and increased SOX4 activity contributes to cellular transformation, cell survival, and metastasis. Gene expression profiling has uncovered SOX4 with upregulated activity during TGF--induced epithelial-mesenchymal transition (EMT) in normal and cancerous breast epithelial cells. SOX4 is indispensable for EMT and cell survival invitro and for primary tumor growth and metastasis invivo. SOX4 is identified as a master regulator of EMT by governing the expression of the epigenetic modifier Ezh2.

Molecular Weight: 47kDa

Gene ID: 6659

UniProt: Q06945