CD147 antibody

Catalog No. ABIN6940674

This Mouse Monoclonal antibody specifically detects CD147 in WB, IHC, FACS and IF. It exhibits reactivity toward Human.

Quick Overview for CD147 antibody (ABIN6940674)

Target: CD147 (BSG) (Basigin (Ok Blood Group) (BSG))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD147 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF)

Clone: 8D6

Product Details anti-CD147 Antibody

Purpose: CD147 / BSG / EMMPRIN / Neurothelin Antibody

Specificity: This MAb recognizes extracellular epitope 2 within the N-terminal Ig domain of human CD147. It is expressed more intensely on thymocytes than on mature peripheral blood T cells. CD147 is important in spermatogenesis, embryo implantation, neural network formation, and tumor progression. It stimulates the production of interstitial collagenase, gelatinase A, stromelysin-1 and various metalloproteinases (MMPs) by fibroblasts. These enzymes are important factors in cancer invasion and metastasis.

Purification: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Human T cell line Molt 13

Isotype: IgG1, kappa

Application Details

Application Notes:

Positive Control: HSB2 cells. Renal Cell, Ovarian carcinoma or Melanoma.

Known Application: Inhibits Homotypic Aggregation, Adhesion to Matrix Proteins, and Migation through Matrigel (Order Ab without BSA/Azide),Flow Cytometry (0.5-1 μg/million cells), Immunofluorescence (1:100-1:200), Immunohistochemistry (Formalin-fixed) (0.5-1 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for CD147

Target: CD147 (BSG) (Basigin (Ok Blood Group) (BSG))

Alternative Name: BSG

Background:

5A11 Antigen, 5F7, Basigin, Blood brain barrier HT7 antigen BSG, Extracellular Matrix Metalloproteinase Inducer (EMMPRIN), Leukocyte Activation Antigen M6, Neurothelin, OK Blood Group Antigen, TCSF, Tumor Cell-derived Collagenase Stimulatory Factor

HepG2, HeLa. Human renal cell carcinoma, ovarian carcinoma or melanoma.

Molecular Weight: 35kDa (non-reduced), 40kDa (reduced)

Gene ID: 682, 501293

UniProt: P35613

Pathways: S100 Proteins