TGFA antibody (C-Term)

Catalog No. ABIN6940715

This Mouse Monoclonal antibody specifically detects TGFA in IHC, IF, ELISA, StM and Coat. It exhibits reactivity toward Human, Rat, Mouse and Cow.

Quick Overview for TGFA antibody (C-Term) (ABIN6940715)

Target: TGFA (Transforming Growth Factor, alpha (TGFA))

Reactivity: Human, Rat, Mouse, Cow

Host: Mouse

Clonality: Monoclonal

Conjugate: This TGFA antibody is un-conjugated

Application: Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA, Staining Methods (StM), Coating (Coat)

Clone: MF9

Product Details anti-TGFA Antibody

Binding Specificity: C-Term

Specificity: This antibody reacts with the C-terminus of TGF alpha and shows no cross-reaction with EGF and the neuropeptide synenkephalin. The staining with Ab-1 is completely blocked by the peptide used for raising this antibody. TGF (aa50) is a growth factor with 33 % homology to EGF, binds to EGFR, activates tyrosine phosphorylation of the receptor, and stimulates cell proliferation. It plays a role in tumor initiation by inducing the reversible transformed phenotype.

Purification: Purified by Protein A/G

Immunogen: A 17-amino acid synthetic peptide from the carboxyl-terminus of rat TGFα (exact sequence is proprietary)

Isotype: IgG1 kappa

Application Details

Application Notes:

Positive Control: Jurkat or Ramos cells. Testicular Carcinoma.

Known Application: ELISA (Use Ab at 2-4 μg/mL for coating) (Order Ab without BSA), Immunofluorescence (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (2-4 μg/mL for 30 min at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for TGFA

Target: TGFA (Transforming Growth Factor, alpha (TGFA))

Alternative Name: TGFA

Molecular Weight: ~6kDa

Gene ID: 7039

UniProt: P01135

Pathways: NF-kappaB Signaling, RTK Signaling, EGFR Signaling Pathway