TGFA antibody (AA 34-43)

Catalog No. ABIN6940717

This anti-TGFA antibody is a Mouse Monoclonal antibody detecting TGFA in IHC, IF, FACS and StM. Suitable for Human, Rabbit and Zebrafish (Danio rerio).

Quick Overview for TGFA antibody (AA 34-43) (ABIN6940717)

Target: TGFA (Transforming Growth Factor, alpha (TGFA))

Reactivity: Human, Rabbit, Zebrafish (Danio rerio)

Host: Mouse

Clonality: Monoclonal

Conjugate: This TGFA antibody is un-conjugated

Application: Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS), Staining Methods (StM)

Clone: 1E8-G6

Product Details anti-TGFA Antibody

Binding Specificity: AA 34-43

Specificity: This antibody reacts with the TGF alpha and shows no cross-reaction with EGF and the neuropeptide synenkephalin. The staining with this MAb is completely blocked by the peptide used for raising this antibody. TGF (aa50) is a growth factor with 33 % homology to EGF, binds to EGFR, activates tyrosine phosphorylation of the receptor, and stimulates cell proliferation. It plays a role in tumor initiation by inducing the reversible transformed phenotype.

Immunogen: A 10-amino acid synthetic peptide (aa34-43; PPVAAAVVSH) from human TGFα.

Isotype: IgM kappa

Application Details

Application Notes:

Positive Control: Jurkat or Ramos cells. Testicular Carcinoma.

Known Application: Flow Cytometry (0.5-1 μg/million cells), Immunofluorescence (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (2-4 μg/mL for 30 min at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for TGFA

Target: TGFA (Transforming Growth Factor, alpha (TGFA))

Alternative Name: TGFA

Molecular Weight: ~6kDa

Gene ID: 7039

UniProt: P01135

Pathways: NF-kappaB Signaling, RTK Signaling, EGFR Signaling Pathway