Thrombomodulin antibody (AA 69-194)

Catalog No. ABIN6940728

This anti-Thrombomodulin antibody is a Mouse Monoclonal antibody detecting Thrombomodulin in IHC, ELISA, Coat, ISt and StM. Suitable for Human.

Quick Overview for Thrombomodulin antibody (AA 69-194) (ABIN6940728)

Target: Thrombomodulin (THBD)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Thrombomodulin antibody is un-conjugated

Application: Immunohistochemistry (IHC), ELISA, Coating (Coat), Immunostaining (ISt), Staining Methods (StM)

Clone: THBD-1782

Product Details anti-Thrombomodulin Antibody

Binding Specificity: AA 69-194

Specificity: It recognizes a protein of 75 kDa, identified as Thrombomodulin. Thrombomodulin is a transmembrane glycoprotein with natural anticoagulant properties. It is normally expressed by a restricted number of cells, such as endothelial and mesothelial cells. In addition, synovial lining and syncytio-trophoblasts of placenta also express thrombomodulin. This protein is present in almost all of benign vascular tumors and majority of malignant vascular tumors (Kaposi's sarcoma, angiosarcoma, and epithelioid hemangioendothelioma). Hence, anti-thrombomodulin serves as a sensitive marker for lymphatic endothelial cells and their tumors. Recently, thrombomodulin antibody has been used for mesothelial cells and malignant mesotheliomas.

Purification: Purified by Protein A/G

Immunogen: Recombinant fragment (around aa 69-194) of human Thrombomodulin (CD141) protein (exact sequence is proprietary)

Isotype: IgG2a kappa

Application Details

Application Notes:

Positive Control: THP1 cells. Placenta, Angiosarcoma, Bladder or Cervical Carcinoma.

Known Application: ELISA (Use Ab at 2-4 μg/mL for coating) (Order Ab without BSA), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM Tris buffer with 1 mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for Thrombomodulin

Target: Thrombomodulin (THBD)

Alternative Name: THBD

Molecular Weight: ~100kDa

Gene ID: 7056

UniProt: P07204