TLE1 antibody (AA 175-338)

Catalog No. ABIN6940747

The Mouse Monoclonal anti-TLE1 antibody is suitable to detect TLE1 in samples from Human. It has been validated for WB, IHC and StM.

Quick Overview for TLE1 antibody (AA 175-338) (ABIN6940747)

Target: TLE1 (Transducin-Like Enhancer of Split 1 (E(sp1) Homolog, Drosophila) (TLE1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This TLE1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Staining Methods (StM)

Clone: TLE1-2051

Product Details anti-TLE1 Antibody

Binding Specificity: AA 175-338

Purification: Purified by Protein A/G

Immunogen: Recombinant human TLE1 fragment (aa 175-338) (exact sequence is proprietary)

Isotype: IgG2a kappa

Application Details

Application Notes:

Positive Control: HeLa, HepG2 or Jurkat cells. Endometrium Carcinoma or Sarcomas.

Known Application: Western Blot (0.5-1 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for TLE1

Target: TLE1 (Transducin-Like Enhancer of Split 1 (E(sp1) Homolog, Drosophila) (TLE1))

Alternative Name: TLE1

Background: Key players in the Notch pathway are the TLE genes, which are human homologs of the Drosophila groucho gene. Groucho is a transcriptional repressor that plays a key role in neurogenesis, segmentation and sex determination. Transducin-like enhancer protein1 (TLE1) is a protein that isencoded by theTLE1 gene and is involved in control of hematopoiesis, neuronal, and terminal epithelial differentiation. Positive immunohistochemical nuclear staining with anti-TLE-1 has been shown to be a useful addition to an IHC panel when differentiating synovial sarcoma from other soft tissue malignancies.

Molecular Weight: 83kDa

Gene ID: 7088

UniProt: Q04724

Pathways: WNT Signaling, SARS-CoV-2 Protein Interactome