TYMS antibody

Catalog No. ABIN6940818

This Mouse Monoclonal antibody specifically detects TYMS in IF, FACS, IHC, ISt, RIA and StM. It exhibits reactivity toward Human.

Quick Overview for TYMS antibody (ABIN6940818)

Target: TYMS (Thymidylate Synthetase (TYMS))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This TYMS antibody is un-conjugated

Application: Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunostaining (ISt), Radioimmunoassay (RIA), Staining Methods (StM)

Clone: TS106

Product Details anti-TYMS Antibody

Specificity: It recognizes a protein of 36 kDa, identified as Thymidylate Synthase (TS) (EC 2.1.1.45). TS converts deoxyuridine monophosphate (dUMP) to deoxythymidine monophosphate (dTMP), which is essential for DNA biosynthesis. TS is also a critical target for the fluoropyrimidines, an important group of antineoplastic drugs that are widely used in the treatment of solid tumors. Both 5-FU and fluorodeoxyuridine are converted in tumor cells to FdUMP which inactivates TS by formation of a ternary covalent complex in the presence of the folate cofactor 5,10-methylenetetrahydrofolate. Expression of TS protein is associated with response to 5-fluorouracil (5-FU) in human colorectal, gastric, head and neck, and breast carcinomas.

Purification: Purified by Protein A/G

Immunogen: Recombinant full-length human TYMS protein.

Isotype: IgG1 kappa

Application Details

Application Notes:

Positive Control: 5-FU-resistant colon carcinoma cell lines (NCI H630R10, NCI H630R1), 5-FU-resistant breast cancer cell lines, MCF-Ad5 and MCF-Ad10. Testicular carcinomas.

Known Application: Flow Cytometry (0.5-1 μg/million cells), Immunofluorescence (0.5-1 μg/mL), ,Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for TYMS

Target: TYMS (Thymidylate Synthetase (TYMS))

Alternative Name: TYMS

Molecular Weight: 36kDa

Gene ID: 7298

UniProt: P04818

Pathways: Mitotic G1-G1/S Phases