TYMS antibody (AA 60-174)

Catalog No. ABIN6940822

Product Details anti-TYMS Antibody

Target: TYMS (Thymidylate Synthetase (TYMS))

Binding Specificity: AA 60-174

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This TYMS antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunostaining (ISt), Radioimmunoassay (RIA), Staining Methods (StM)

Specificity: It recognizes a protein of 36 kDa, identified as Thymidylate Synthase (TS) (EC 2.1.1.45). It converts deoxyuridine monophosphate (dUMP) to deoxythymidine monophosphate (dTMP), which is essential for DNA biosynthesis. TS is also a critical target for the fluoropyrimidines, an important group of antineoplastic drugs that are widely used in the treatment of solid tumors. Both 5-FU and fluorodeoxyuridine are converted in tumor cells to FdUMP which inactivates TS by formation of a ternary covalent complex in the presence of the folate cofactor 5,10-methylenetetrahydrofolate. Expression of TS protein has been reported to associate with response to 5-fluorouracil (5-FU) in human colorectal, gastric, head and neck, and breast carcinomas.

Purification: Purified by Protein A/G

Immunogen: Recombinant human thymidylate synthase protein fragment (around aa 60-174) (exact sequence is proprietary)

Clone: TYMS-1884

Isotype: IgG2c kappa

Application Details

Application Notes:

Positive Control: MOLT4, Ramos, 5-FU-resistant colon carcinoma cell lines (NCI H630R10, NCI H630R1), 5-FU-resistant breast cancer cell lines, MCF-Ad5 and MCF-Ad10. Testicular carcinomas.

Known Application: Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Western Blot (1-2 μg/mL), ,Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody is stable for 24 months. ,Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for TYMS

Target: TYMS (Thymidylate Synthetase (TYMS))

Alternative Name: TYMS (TYMS Products)

Synonyms: FN1 antibody, ECK2823 antibody, JW2795 antibody, ts antibody, Ts antibody, HST422 antibody, TMS antibody, TS antibody, thymidylate synthetase antibody, contains intron in T4 phage antibody, ThyE antibody, thymidylate synthase antibody, thymidylate synthetase L homeolog antibody, TYMS antibody, thyA antibody, td antibody, thyE antibody, DDA3937_RS04960 antibody, tyms.L antibody, XBJ1_RS15770 antibody, EAMY_RS20680 antibody, Tyms antibody

Target Type: Viral Protein

Molecular Weight: 36kDa

Gene ID: 7298

UniProt: P04818

Pathways: Mitotic G1-G1/S Phases