Recombinant Villin 1 antibody (AA 179-311)

Catalog No. ABIN6940874

This Mouse Monoclonal antibody specifically detects Villin 1 in IHC and StM. It exhibits reactivity toward Human.

Quick Overview for Recombinant Villin 1 antibody (AA 179-311) (ABIN6940874)

Target: Villin 1 (VIL1)

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Villin 1 antibody is un-conjugated

Application: Immunohistochemistry (IHC), Staining Methods (StM)

Clone: RVIL1-1325

Product Details anti-Villin 1 Antibody

Binding Specificity: AA 179-311

Specificity: Recognizes a protein of 95 kDa, which is identified as villin. It is a major constituent in the microvilli, which compose the brush border of epithelial cells forming absorptive surfaces of the intestinal and renal proximal tubular epithelia. Anti-Villin labels the brush border area in the gastrointestinal mucosal epithelium and urogenital tract. Among neoplasms, villin is predominantly expressed in tumors of colorectal origin. Antibody to villin is useful in identifying malignant cells from primary and metastatic colorectal carcinomas. This antibody also labels Merkel cells of the skin.

Purification: Purified by Protein A/G

Immunogen: A recombinant fragment (around aa179-311) of human Villin protein (exact sequence is proprietary)

Isotype: IgG1 kappa

Application Details

Application Notes:

Positive Control: A549, HepG2 and HCT116 cells. Colon or Rectum.

Known Application: Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for Villin 1

Target: Villin 1 (VIL1)

Alternative Name: VIL1

Molecular Weight: 93kDa

Gene ID: 7429

UniProt: P09327

Pathways: EGFR Signaling Pathway, Regulation of Actin Filament Polymerization