Caldesmon antibody

Catalog No. ABIN6940940

This Mouse Monoclonal antibody specifically detects Caldesmon in WB and IHC. It exhibits reactivity toward Human.

Quick Overview for Caldesmon antibody (ABIN6940940)

Target: Caldesmon (CALD1) (Caldesmon 1 (CALD1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Caldesmon antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Clone: CALD1-820

Product Details anti-Caldesmon Antibody

Purpose: Caldesmon, HMW (h-Caldesmon) (Smooth Muscle Marker) Antibody

Specificity: Recognizes a protein of 150kDa, which is identified as the high molecular weight variant of Caldesmon. Two closely related variants of human caldesmon have been identified which are different in their electrophoretic mobility and cellular distribution. The h-caldesmon variant (120'150kDa) is predominantly expressed in smooth muscle whereas l-caldesmon (70'80kDa) is found in non- muscle tissue and cells. Neither of the two variants has been detected in skeletal muscle. This MAb recognizes only the 150kDa variant (h-caldesmon) in Western blots of human aortic media extracts and is unreactive with fibroblast extracts from cultivated human foreskin. Caldesmon is a developmentally regulated protein involved in smooth muscle and non-muscle contraction.

No Cross-Reactivity: Rat (Rattus)

Purification: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Recombinant full-length human CALD1 protein

Isotype: IgG1, kappa

Application Details

Application Notes:

Positive Control: Uterus, Blood vessels in all tissues, smooth muscle or leiomyosarcoma.

Known Application: Immunohistochemistry (Formalin-fixed) (0.25-0.5 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 1 mM EDTA, pH 7.5-8.5, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for Caldesmon

Target: Caldesmon (CALD1) (Caldesmon 1 (CALD1))

Alternative Name: CALD1

Background:

CAD, CALD1, Caldesmon 1 Isoform 1, Caldesmon 1 Isoform 2, Caldesmon 1 Isoform 3, Caldesmon 1 Isoform 4, Caldesmon 1 Isoform 5, CDM, HCAD, LCAD, NAG22

Blood vessels in all tissues, smooth muscle or leiomyosarcoma., Uterus

Molecular Weight: 150kDa

Gene ID: 800, 490203

UniProt: Q05682

Pathways: Myometrial Relaxation and Contraction