Cyclin E1 antibody (AA 10-176)

Catalog No. ABIN6940995

This Mouse Monoclonal antibody specifically detects Cyclin E1 in ELISA and Coat. It exhibits reactivity toward Human.

Quick Overview for Cyclin E1 antibody (AA 10-176) (ABIN6940995)

Target: Cyclin E1 (CCNE1)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Cyclin E1 antibody is un-conjugated

Application: ELISA, Coating (Coat)

Clone: CCNE1-2587

Product Details anti-Cyclin E1 Antibody

Binding Specificity: AA 10-176

Purification: Purified by Protein A/G

Immunogen: Recombinant human Cyclin E (CCNE1) protein fragment (around aa 10-176) (exact sequence is proprietary)

Isotype: IgG kappa

Application Details

Application Notes:

Positive Control: HeLa cells. Breast or Colon Carcinoma.

Known Application: ELISA (For coating, order Ab without BSA), Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for Cyclin E1

Target: Cyclin E1 (CCNE1)

Alternative Name: CCNE1

Background: Cyclin E belongs to the highly conserved cyclin family, whose members exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. Cyclins function as regulators of CDK kinases. Cyclin E forms a complex with and functions as a regulatory subunit of CDK2, whose activity is required for cell cycle G1/S transition. Cyclin E accumulates at the G1-S phase boundary and is degraded as cells progress through S phase. Cyclin E overexpression has been observed in many tumors, which results in chromosome instability, and thus may contribute to tumorigenesis.

Molecular Weight: 53kDa

Gene ID: 898

UniProt: P24864

Pathways: Cell Division Cycle, Intracellular Steroid Hormone Receptor Signaling Pathway, Nuclear Hormone Receptor Binding, Mitotic G1-G1/S Phases